518 .
GUYPARKERIA MEDIUM
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Equipment needed:
Autoclave
Filter
Final pH:
7.3 - 7.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2HPO4 x 2 H2O | 7.9 | g | ||
|
|
KH2PO4 | 1.5 | g | ||
|
|
NaCl | 50.0 | g | ||
|
|
MgSO4 x 7 H2O | 0.1 | g | ||
|
|
NH4Cl | 0.4 | g | ||
|
|
Phenol red
(saturated aq. solution) |
12.5 | ml | ||
|
|
Trace element solution | 10.0 | ml | ||
|
|
Na2S2O3 x 5 H2O | 5.0 | g | ||
|
|
Agar
, for solid medium
optional |
15.0 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Dissolve ingredients except thiosulfate and adjust pH of medium to 7.3 - 7.5. Add thiosulfate to the medium after autoclaving from a sterile stock solution sterilized by filtration. For liquid medium also sterilize the phosphates separately in 1/10 of medium volume. | |||||
Trace element solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2-EDTA | 50.00 | g | ||
|
|
ZnSO4 x 7 H2O | 11.00 | g | ||
|
|
CaCl2 x 2 H2O | 7.34 | g | ||
|
|
MnCl2 x 4 H2O | 2.50 | g | ||
|
|
CoCl2 x 6 H2O | 0.50 | g | ||
|
|
(NH4)6Mo7O24 x 4 H2O | 0.50 | g | ||
|
|
FeSO4 x 7 H2O | 5.00 | g | ||
|
|
CuSO4 x 5 H2O | 0.20 | g | ||
|
|
NaOH | 11.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. Adjust pH of final solution to 6.0 with NaOH. | |||||