MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 486 . THIOBACILLUS THIOPARUS (TK-m) MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Thiobacillus thioparus DSM 5368

This strain uses the standard medium without modifications.

Growth conditions: 2-3 days 30 °C

Main sol. 486 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 2.0 g 2 14.697
K2HPO4 2.0 g 2 11.483
NH4Cl 0.4 g 0.4 7.478
Na2CO3 0.4 g 0.4 3.774
MgCl2 x 6 H2O 0.2 g 0.2 0.984
Vitamin solution 3.0 ml - -
Trace element solution 1.0 ml - -
Bromocresol purple
(saturated aq. solution) 		2.0 ml - -
Na2S2O3 x 5 H2O 5.0 g 5 20.146
Agar 15.0 g 15 44.599
Distilled water 1000.0 ml - -
1 Sterilize the phosphates separately in 1/10 of medium volume and mix with the other salts when cool. Add filter-sterilized vitamin solution after autoclaving to sterile medium. Adjust pH of complete medium to 7.1, if necessary.

Vitamin solution #

1000
Solution occurs in 1 other media (first defined in Medium 484).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Thiamine-HCl x 2 H2O 10 mg 0.01 0.027
Nicotinic acid 20 mg 0.02 0.162
Pyridoxine hydrochloride 20 mg 0.02 0.097
p-Aminobenzoic acid 10 mg 0.01 0.073
Riboflavin 20 mg 0.02 0.053
Calcium pantothenate 20 mg 0.02 0.042
Biotin 1 mg 1.0e-3 0.004
Vitamin B12 1 mg 1.0e-3 7.4e-4
Distilled water 1000 ml - -
1 Adjust pH to 7.0.

Trace element solution #

1000
Solution occurs in 5 other media (first defined in Medium 333).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 50.00 g 50 148.718
ZnSO4 x 7 H2O 11.00 g 11 38.253
CaCl2 x 2 H2O 7.34 g 7.34 49.927
MnCl2 x 4 H2O 2.50 g 2.5 12.632
CoCl2 x 6 H2O 0.50 g 0.5 3.851
(NH4)6Mo7O24 x 4 H2O 0.50 g 0.5 -
FeSO4 x 7 H2O 5.00 g 5 17.985
CuSO4 x 5 H2O 0.20 g 0.2 0.801
NaOH 11.00 g 11 275.021
Distilled water 1000.00 ml - -
1 Dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. Adjust pH of final solution to 6.0 with NaOH.

Metadata

Last modified: 28.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.1
Equipment needed: Autoclave, Filter
Edit in Medium builder

Associated strains

All strains for this medium: 2
Thiobacillus thioparus DSM 5368

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Na2S2O3 x 5 H2O 5 20.146
KH2PO4 2 14.697
K2HPO4 2 11.483
NH4Cl 0.4 7.478
Show more
CSV
JSON

Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
temperature mesophilic 2 strains