MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 484 . THIOMICROSPIRA THYASIRAE MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Thiomicrospira thyasirae DSM 5322

This strain uses the standard medium without modifications.

Growth conditions: 30 °C

Main sol. 484 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 100 ml - -
Solution B 860 ml - -
Solution C 10 ml - -
Solution D 20 ml - -
Solution E 10 ml - -
1 Autoclave solutions A, B and C separately and mix when cool. After autoclaving, add thiosulfate, trace elements and vitamins from sterile stock solutions to complete the medium. Adjust pH of complete medium to 7.3 - 7.6.

Solution A #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 7.9 g 79 556.499
KH2PO4 1.5 g 15 110.225
Distilled water 100.0 ml - -
1 Adjust to pH 7.6.

Solution B #

860
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.4 g 0.465 8.695
MgSO4 x 7 H2O 0.1 g 0.116 0.472
NaCl 25.0 g 29.07 497.429
Agar , for solid medium 15.0 g 17.442 51.859
Distilled water 860.0 ml - -

Solution C #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trace element solution 10 ml - -

Solution D #

20
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S2O3 x 5 H2O 5 g 250 1007.31
Distilled water 20 ml - -

Solution E #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin solution 10 ml - -

Vitamin solution #

1000
Solution occurs in 1 other media (first defined in Medium 484).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Thiamine-HCl x 2 H2O 10 mg 0.01 0.027
Nicotinic acid 20 mg 0.02 0.162
Pyridoxine hydrochloride 20 mg 0.02 0.097
p-Aminobenzoic acid 10 mg 0.01 0.073
Riboflavin 20 mg 0.02 0.053
Calcium pantothenate 20 mg 0.02 0.042
Biotin 1 mg 1.0e-3 0.004
Vitamin B12 1 mg 1.0e-3 7.4e-4
Distilled water 1000 ml - -
1 Adjust pH to 7.0.

Trace element solution #

1000
Solution occurs in 5 other media (first defined in Medium 333).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 50.00 g 50 148.718
ZnSO4 x 7 H2O 11.00 g 11 38.253
CaCl2 x 2 H2O 7.34 g 7.34 49.927
MnCl2 x 4 H2O 2.50 g 2.5 12.632
CoCl2 x 6 H2O 0.50 g 0.5 3.851
(NH4)6Mo7O24 x 4 H2O 0.50 g 0.5 -
FeSO4 x 7 H2O 5.00 g 5 17.985
CuSO4 x 5 H2O 0.20 g 0.2 0.801
NaOH 11.00 g 11 275.021
Distilled water 1000.00 ml - -
1 Dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. Adjust pH of final solution to 6.0 with NaOH.

Metadata

Last modified: 28.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.3 - 7.6
Equipment needed: Autoclave, Filter
Edit in Medium builder

Associated strains

All strains for this medium: 1
Thiomicrospira thyasirae DSM 5322

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 25 427.789
Agar 15 44.599
Na2HPO4 x 2 H2O 7.9 55.65
Na2S2O3 x 5 H2O 5 20.146
KH2PO4 1.5 11.023
Show more
CSV
JSON

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain