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DSMZ

DSMZ 403 . BSK-MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Borrelia bissettiae DSM 17990

This strain uses the standard medium without modifications.

Growth conditions: 35 °C

Main sol. 403 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 1000 ml - -
Solution B 200 ml - -
Solution C 143 ml - -
Solution D 86 ml - -
1 Combine the warm (37°C) solutions A, B, C, D, and filter-sterilize the warm medium. Solution B can also be added after filtration.
2 Alternatively, buy supplemented or un-supplemented BSK-H medium (Fa. Bio&SELL or Sigma) and supplement the medium according to manufacturer's advice. Sterilize the mixed and warm (37°C) medium by filtration.

Solution A #

1000
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
CMRL 1066
(GIBCO 042-1545, glutamin 10 x) 		100.0 ml - -
Proteose peptone no. 2
(DIFCO 0121-01-3) 		5.0 g 5 -
Tryptone peptone
(DIFCO 0123-01) 		1.0 g 1 -
Proteose yeastolate
(TC) (DIFCO 5577-15) 		1.0 g 1 -
HEPES buffer
(acid) (SIGMA H3375) 		6.0 g 6 25.178
Sodium citrate 0.7 g 0.7 2.712
Glucose
(alpha-, D+) 		3.0 g 3 16.652
Na-pyruvate 0.8 g 0.8 7.27
N-Acetylglucosamine 0.4 g 0.4 1.808
MgCl2 x 6 H2O 0.3 g 0.3 1.476
L-Glutamine 0.1 g 0.1 0.684
Double distilled water
(filter-sterilized) 		900.0 ml - -
1 Stir slowly at 4 - 10°C for 3 hours, adjust pH to 7.6 with 5 M NaOH. Filter sterilize.

Solution B #

200
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Gelatine 14 g 70 -
Double distilled water 200 ml - -
1 Dissolve 14.0 g gelatine in 200 ml bidest. water, autoclave 15 minutes at 115°C.

Solution C #

143
Compound Amount Unit Conc. [g/L] Conc. [mM]
Bovine serum albumin
(Sigma No A9647) 		50.05 g 350 -
Double distilled water 143.00 ml - -
1 Dissolve 50.05 g bovine serum albumine, fract. V (important: Sigma No A9647) in 143 ml bidest. water, or use BSA-solution Sigma A7409.

Solution D #

86
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Rabbit serum
(inactivated, GIBCO 037-06120M) 		86 ml - 526320

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 23
Strains with modifications: 4
Borrelia bissettiae DSM 17990

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Bovine serum albumin 35.025 -
Gelatine 9.797 -
HEPES buffer 4.199 17.62
Proteose peptone no. 2 3.499 -
Glucose 2.099 11.653
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Cultivation metadata from 23 strains BacDive

phyla 2 23 strains
oxygen microaerophile 18 strains
temperature mesophilic 23 strains