Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
CMRL 1066
(GIBCO 042-1545, glutamin 10 x) |
100.0 | ml | - | - | |
|
Proteose peptone no. 2
(DIFCO 0121-01-3) |
5.0 | g | 5 | - | |
|
Tryptone peptone
(DIFCO 0123-01) |
1.0 | g | 1 | - | |
|
Proteose yeastolate
(TC) (DIFCO 5577-15) |
1.0 | g | 1 | - | |
|
HEPES buffer
(acid) (SIGMA H3375) |
6.0 | g | 6 | 25.178 | |
| Sodium citrate | 0.7 | g | 0.7 | 2.712 | |
|
Glucose
(alpha-, D+) |
3.0 | g | 3 | 16.652 | |
| Na-pyruvate | 0.8 | g | 0.8 | 7.27 | |
| N-Acetylglucosamine | 0.4 | g | 0.4 | 1.808 | |
| MgCl2 x 6 H2O | 0.3 | g | 0.3 | 1.476 | |
| L-Glutamine | 0.1 | g | 0.1 | 0.684 | |
|
Double distilled water
(filter-sterilized) |
900.0 | ml | - | - | |
| 1 Stir slowly at 4 - 10°C for 3 hours, adjust pH to 7.6 with 5 M NaOH. Filter sterilize. | |||||
Media with this solution
| 403 | BSK-MEDIUM |