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DSMZ

DSMZ 3 . AZOTOBACTER MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Medium for Azotobacter vinelandii DSM 399

This strain uses the standard medium without modifications.

Growth conditions: 30 °C

Main sol. 3 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Glucose 5.00 g 5 27.753
Mannitol 5.00 g 5 27.447
CaCl2 x 2 H2O 0.10 g 0.1 0.68
MgSO4 x 7 H2O 0.10 g 0.1 0.406
Na2MoO4 x 2 H2O 5.00 mg 0.005 0.021
K2HPO4 0.90 g 0.9 5.167
KH2PO4 0.10 g 0.1 0.735
FeSO4 x 7 H2O 0.01 g 0.01 0.036
CaCO3 5.00 g 5 49.955
Agar 15.00 g 15 44.599
Distilled water 950.00 ml - -
1 Adjust pH to 7.3.
2 Sterilize glucose and mannitol separately (in 50 ml H2O) and add to the medium after autoclaving. Calcium carbonate in the medium serves as a buffer. The calcium carbonate will settle in agar plates before the agar has set, producing an opaque layer in the bottom. As the strain grows and acid is produced this will react with the calcium carbonate, causing it to dissolve and form zones of clearing immediately below the colonies.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.3
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 40
Azotobacter vinelandii DSM 399

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
CaCO3 5 49.955
Glucose 5 27.753
Mannitol 5 27.447
K2HPO4 0.9 5.167
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Cultivation metadata from 40 strains BacDive

phyla 1 40 strains
temperature mesophilic 39 strains