396
396 .
METHANOSALSUM ZHILINAE MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Methanosalsum zhilinae DSM 4017
This strain uses the standard medium without modifications.
Growth conditions: anaerobic
45 °C
Main sol. 396 #
ml
Equipment needed: Hungate tubes
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Yeast extract
(OXOID) |
2.00 | g | 2 | - | |
|
Trypticase peptone
(BD BBL) |
2.00 | g | 2 | - | |
| NaCl | 40.00 | g | 40 | 684.463 | |
| NH4Cl | 1.00 | g | 1 | 18.695 | |
| MgCl2 x 6 H2O | 3.50 | g | 3.5 | 17.216 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| KCl | 1.00 | g | 1 | 13.414 | |
| K2HPO4 | 0.40 | g | 0.4 | 2.297 | |
| Modified Wolin's mineral solution | 5.00 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.5 | 2.847 | |
| NaHCO3 | 3.00 | g | 3 | 35.711 | |
| Na2CO3 | 2.00 | g | 2 | 18.87 | |
| Trimethylamine-HCl | 2.00 | g | 2 | - | |
| Na2S x 9 H2O | 0.25 | g | 0.25 | 1.041 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except cysteine, bicarbonate, carbonate, trimethylamine and sulfide, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add cysteine and dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add trimethylamine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas, whereas bicarbonate and carbonate are added from sterile anoxic stock solutions prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust pH of complete medium to 9.2 - 9.4. | |||||
Solution occurs in 167
other media (first defined in Medium 141).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| MnSO4 x H2O | 0.50 | g | 0.5 | 2.958 | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.18 | g | 0.18 | 1.161 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.18 | g | 0.18 | 0.626 | |
| CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
| AlK(SO4)2 x 12 H2O | 0.02 | g | 0.02 | 0.042 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 | |
| Na2SeO3 x 5 H2O | 0.30 | mg | 3.0e-4 | 0.001 | |
| Na2WO4 x 2 H2O | 0.40 | mg | 4.0e-4 | 0.001 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. | |||||
Metadata
| Last modified: | 28.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Archaea |
| Medium type: | Complex medium |
| Final pH: | 9.2 - 9.4 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Methanosalsum zhilinae DSM 4017
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| MgCl2 x 6 H2O | 3.5 | 17.216 |
| NaHCO3 | 3 | 35.711 |
| Na2CO3 | 2 | 18.87 |
| Trypticase peptone | 2 | - |
| Trimethylamine-HCl | 2 | - |
| Show more | ||
