38
38 .
ACETOANAEROBIUM MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Acetoanaerobium sticklandii DSM 519
This strain uses the standard medium without modifications.
Growth conditions: anaerobic
35 °C
Main sol. 38 #
ml
Equipment needed: Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Tryptone | 20.00 | g | 20 | 281.373 | |
| Yeast extract | 10.00 | g | 10 | - | |
| K2HPO4 | 1.04 | g | 1.04 | 5.971 | |
| KH2PO4 | 0.68 | g | 0.68 | 4.997 | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
| Na2S x 9 H2O | 0.15 | g | 0.15 | 0.625 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except sulfide), adjust pH to 7.0, bring medium to the boil, then cool to room temperature under 100% N2 gas. Dispense under same gas atmosphere in culture vessels and autoclave. After sterilization add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave, Gassing station |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Acetoanaerobium sticklandii DSM 519
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Tryptone | 20 | 281.373 |
| Yeast extract | 10 | - |
| K2HPO4 | 1.04 | 5.971 |
| KH2PO4 | 0.68 | 4.997 |
| Na2S x 9 H2O | 0.15 | 0.625 |
| Show more | ||
