339
339 .
WILKINS-CHALGREN ANAEROBE BROTH
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Enterococcus faecalis DSM 106192
* Modification: Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT).
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 1-2 days
anaerobic
37 °C
pH 7.00
Main sol. 339 #
ml
Equipment needed: Filter
Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
dehydrated Wilkins-Chalgren medium
(Oxoid CM0643) |
33.00 | g | 33 | - | |
|
Sodium resazurin*
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| L-Cysteine HCl | 0.30 | g | 0.3 | 1.903 | |
| Phenosafranine* | 2.00 | ml/l | - | - | |
| Cystein* | 0.05 | g/ml | - | - | |
| DTT* | 0.02 | g/ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve 33.0 g/l dehydrated Wilkins-Chalgren medium (Oxoid CM0643) in distilled water and add 0.5 ml/l Na-resazurin solution (0.1% w/v), bring to the boil and cool to room temperature while sparging with 100% N2 gas. Add L-cysteine-HCL x H2O (0.3 g/l), dispense the medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving check pH and adjust to pH 6.8 - 7.0, if necessary. | |||||
| 2 Note: In case resazurin is not colourless addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) can be used to completely reduce the medium. | |||||
Metadata
| Last modified: | 21.09.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.8 - 7.0 |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| dehydrated Wilkins-Chalgren medium | 33 | - |
| L-Cysteine HCl | 0.3 | 1.903 |
| Sodium resazurin | 5.0e-4 | 0.002 |
| Distilled water | - | - |
