{"medium":{"id":339,"name":"WILKINS-CHALGREN ANAEROBE BROTH","complex_medium":"yes","min_pH":6.8,"max_pH":7,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium339.pdf"},"solutions":[{"id":651,"name":"Main sol. 339","volume":1000,"recipe":[{"recipe No.":1,"compound":"dehydrated Wilkins-Chalgren medium","compound_id":2095,"attribute":"Oxoid CM0643","amount":33,"unit":"g","g_l":33,"optional":"no"},{"recipe No.":2,"compound":"Sodium resazurin","compound_id":1852,"attribute":"0.1% w/v","amount":0.5,"unit":"ml","g_l":0.0005,"mmol_l":0.00199068,"optional":"no"},{"recipe No.":3,"compound":"L-Cysteine HCl","compound_id":1157,"amount":0.3,"unit":"g","g_l":0.3,"mmol_l":1.90331,"optional":"no"},{"recipe No.":4,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve 33.0 g/l dehydrated Wilkins-Chalgren medium (Oxoid CM0643) in distilled water and add 0.5 ml/l Na-resazurin solution (0.1% w/v), bring to the boil and cool to room temperature while sparging with 100% N2 gas. Add L-cysteine-HCL x H2O (0.3 g/l), dispense the medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving check pH and adjust to pH 6.8 - 7.0, if necessary."},{"step":"Note: In case resazurin is not colourless addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) can be used to completely reduce the medium."}],"equipment":{"1072":"Filter","3068":"Autoclave","3874":"Hungate tubes","4277":"Gassing station"},"main solution":"yes"}]}