330
330 .
RUMEN BACTERIA MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Eshraghiella crossota DSM 2876
This strain uses the standard medium without modifications.
Growth conditions: anaerobic
37 °C
Main sol. 330 #
ml
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Mineral solution | 38.00 | ml | - | - | |
| K2HPO4 | 0.30 | g | 0.3 | 1.722 | |
|
Trypticase peptone
(BD BBL) |
2.00 | g | 2 | - | |
|
Yeast extract
(OXOID) |
0.50 | g | 0.5 | - | |
| Volatile fatty acid mixture | 3.10 | ml | - | - | |
|
Haemin solution
(0.05% w/v) |
2.00 | ml | - | - | |
| Glycerol | 0.50 | g | 0.5 | 5.429 | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
| Na2CO3 | 4.00 | g | 4 | 37.74 | |
| D-Glucose | 0.50 | g | 0.5 | 2.775 | |
| Maltose | 0.50 | g | 0.5 | 1.461 | |
| Cellobiose | 0.50 | g | 0.5 | 1.461 | |
| Starch , soluble | 0.50 | g | 0.5 | - | |
| L-Cysteine HCl x H2O | 0.25 | g | 0.25 | 1.423 | |
| Na2S x 9 H2O | 0.25 | g | 0.25 | 1.041 | |
| Distilled water | 960.00 | ml | - | - | |
| 1 Dissolve ingredients (except carbonate, glucose, maltose, cellobiose, soluble starch, cysteine and sulfide), then sparge medium with 100% CO2 gas for 30 - 45 min to make it anoxic. Add the carbonate and equilibrate the medium with the CO2 gas to pH 6.8. Distribute under 100% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Thereafter, add glucose, maltose, cellobiose, soluble starch, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Adjust pH of complete medium to 6.7 - 6.8, if necessary. | |||||
Solution occurs in 4
other media (first defined in Medium 330).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| KH2PO4 | 6.0 | g | 6 | 44.09 |
| NaCl | 12.0 | g | 12 | 205.339 |
| (NH4)2SO4 | 6.0 | g | 6 | 45.406 |
| CaCl2 x 2 H2O | 1.6 | g | 1.6 | 10.883 |
| MgSO4 x 7 H2O | 2.5 | g | 2.5 | 10.143 |
| Distilled water | 1000.0 | ml | - | - |
Solution occurs in 4
other media (first defined in Medium 330).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Acetic acid | 548.50 | ml | 573.182 | 9545.09 |
| Propionic acid | 193.50 | ml | 192.145 | 2593.76 |
| Butyric acid | 129.00 | ml | - | - |
| n-Valeric acid | 32.25 | ml | 30.283 | 296.512 |
| iso-Butyric acid | 32.25 | ml | 30.638 | 347.719 |
| DL-2-Methylbutyric acid | 32.25 | ml | - | - |
| iso-Valeric acid | 32.25 | ml | 30.025 | 293.986 |
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Haemin | 50 | mg | 0.5 | 0.767 | |
|
NaOH
(1 N) |
1 | ml | - | - | |
| Distilled water | 100 | ml | - | - | |
| 1 Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated. | |||||
Metadata
| Last modified: | 25.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.7 - 6.8 |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 27 |
| Strains with modifications: | 1 |
Eshraghiella crossota DSM 2876
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Na2CO3 | 4 | 37.74 |
| Trypticase peptone | 2 | - |
| Glycerol | 0.5 | 5.429 |
| D-Glucose | 0.5 | 2.775 |
| Maltose | 0.5 | 1.461 |
| Show more | ||
Cultivation metadata from 27 strains
| pH | 6 - 8 | 1 strain |
| phyla | 3 | 24 strains |
| nutrition | chemoorganoheterotroph | 1 strain |
| oxygen | anaerobe | 11 strains |
| oxygen | obligate anaerobe | 2 strains |
| Show more | ||