315 .
BACTEROIDES CELLULOSOLVENS MEDIUM
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Equipment needed:
Filter
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NH4Cl | 0.68 | g | ||
|
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K2HPO4 | 0.30 | g | ||
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KH2PO4 | 0.18 | g | ||
|
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(NH4)2SO4 | 0.15 | g | ||
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MgSO4 x 7 H2O | 0.12 | g | ||
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CaCl2 x 2 H2O | 0.06 | g | ||
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FeSO4 x 7 H2O | 0.02 | g | ||
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Trace element solution | 10.00 | ml | ||
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Vitamin solution | 10.00 | ml | ||
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Cellobiose
(cellulose: i.e. MN 300) |
5.00 | g | ||
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Resazurin | 1.00 | mg | ||
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NaHCO3 | 2.00 | g | ||
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L-Cysteine HCl x H2O | 0.25 | g | ||
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Na2S x 9 H2O | 0.25 | g | ||
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Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 7.0. Gas atmosphere: 80% N2 + 20% CO2. | |||||
| 2 Filter sterilize cellobiose separately. | |||||
Trace element solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid | 1.500 | g | ||
|
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MgSO4 x 7 H2O | 3.000 | g | ||
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MnSO4 x H2O | 0.500 | g | ||
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NaCl | 1.000 | g | ||
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FeSO4 x 7 H2O | 0.100 | g | ||
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CoSO4 x 7 H2O | 0.180 | g | ||
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CaCl2 x 2 H2O | 0.100 | g | ||
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ZnSO4 x 7 H2O | 0.180 | g | ||
|
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CuSO4 x 5 H2O | 0.010 | g | ||
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AlK(SO4)2 x 12 H2O | 0.020 | g | ||
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H3BO3 | 0.010 | g | ||
|
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Na2MoO4 x 2 H2O | 0.010 | g | ||
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NiCl2 x 6 H2O | 0.025 | g | ||
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Na2SeO3 x 5 H2O | 0.300 | mg | ||
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Distilled water | 1000.000 | ml | ||
| 1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH). | |||||
Vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
|
Pyridoxine hydrochloride | 10.0 | mg |
|
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Thiamine-HCl x 2 H2O | 5.0 | mg |
|
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Riboflavin | 5.0 | mg |
|
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Nicotinic acid | 5.0 | mg |
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D-Calcium pantothenate | 5.0 | mg |
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Vitamin B12 | 0.1 | mg |
|
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p-Aminobenzoic acid | 5.0 | mg |
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Lipoic acid | 5.0 | mg |
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Distilled water | 1000.0 | ml |