276 .
HALOMONAS MEDIUM
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* Medium for Marinobacter nauticus DSM 11845
But use 50 g/l NaCl instead of 80 g/l NaCl
Growth conditions: 30 °C
Equipment needed:
Final pH:
7.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 50.00 | g/l | ||
|
|
Casamino acids
(with vitamins) |
7.50 | g | ||
|
|
Proteose peptone no. 3 | 5.00 | g | ||
|
|
Yeast extract | 1.00 | g | ||
|
|
Na3-citrate | 3.00 | g | ||
|
|
MgSO4 x 7 H2O | 20.00 | g | ||
|
|
K2HPO4 | 0.50 | g | ||
|
|
Fe(NH4)2(SO4)2 x 6 H2O | 0.05 | g | ||
|
|
Agar , if necessary | 15.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Before autoclaving adjust pH to 7.5 with NaOH | |||||