252
252 .
STARCH - MINERAL SALT - AGAR (STMS)
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
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Medium for Streptomyces staurosporininus DSM 42057
This strain uses the standard medium without modifications.
Growth conditions: 3-7 days
aerobic
28 °C
pH 7.20
Main sol. 252 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Starch
(soluble) |
10 | g | 10 | - | |
| (NH4)2SO4 | 2 | g | 2 | 15.136 | |
| K2HPO4 | 1 | g | 1 | 5.741 | |
| MgSO4 x 7 H2O | 1 | g | 1 | 4.057 | |
| NaCl | 1 | g | 1 | 17.112 | |
| CaCO3 | 2 | g | 2 | 19.982 | |
| Trace element solution | 1 | ml | - | - | |
| Agar | 20 | g | 20 | - | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust pH to 7.2. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| FeSO4 x 7 H2O | 0.1 | g | 1 | 3.597 |
| MnCl2 x 4 H2O | 0.1 | g | 1 | 5.053 |
| ZnSO4 x 7 H2O | 0.1 | g | 1 | 3.478 |
| Distilled water | 100.0 | ml | - | - |
Metadata
| Last modified: | 08.04.24 |
| Source: | DSMZ |
| Synonyms: | ISP 4 Medium |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 626 |
| Strains with modifications: | 20 |
Streptomyces staurosporininus DSM 42057
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 20 | - |
| Starch | 10 | - |
| CaCO3 | 2 | 19.982 |
| (NH4)2SO4 | 2 | 15.136 |
| NaCl | 1 | 17.112 |
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Cultivation metadata from 626 strains
| halophily | non-halophilic | 1 strain |
| pH | 6 - 8 | 49 strains |
| pH | 4 - 6 | 45 strains |
| pH | 8 - 10 | 35 strains |
| pH | 10 - 12 | 18 strains |
| Show more | ||