251
251 .
PEPTONE - MEAT EXTRACT - SOIL EXTRACT AGAR (PFE)
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
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Medium for Mycobacterium kansasii DSM 43224
This strain uses the standard medium without modifications.
Growth conditions: 28 °C
Main sol. 251 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Proteose peptone no. 3 | 5 | g | 5 | - | |
| Meat extract | 3 | g | 3 | - | |
| Glycerol | 20 | g | 20 | 217.179 | |
| Soil extract | 150 | ml | - | - | |
| Agar | 20 | g | 20 | - | |
| Distilled water | 850 | ml | - | - | |
| 1 Adjust pH to 7.0. Soil extract is prepared by sieving air dried garden soil through a coarse sieve and autoclaving 400 g with 960 ml of distilled water at 121°C for one hour. After the mixture is cool and settled, the supernatant is carefully decanted, filtered through paper, autoclaved in 200 ml quantities, and stored at room temperature until cleared by sedimentation. | |||||
Metadata
| Last modified: | 19.09.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 9 |
Mycobacterium kansasii DSM 43224
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Glycerol | 20 | 217.179 |
| Agar | 20 | - |
| Proteose peptone no. 3 | 5 | - |
| Meat extract | 3 | - |
| Soil extract | - | - |
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