1809 .
DC-8A Medium
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Equipment needed:
Anaerobic workbench
Autoclave
Final pH:
9.0
Select a strain (optional):
Main sol. 1809 #
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
K2HPO4 | 2.90 | g | ||
|
|
Urea | 4.20 | g | ||
|
|
Yeast extract | 2.00 | g | ||
|
|
CaCl2 x 2 H2O | 0.01 | g | ||
|
|
Na2CO3 | 1.00 | g | ||
|
|
L-Cysteine HCl x H2O | 0.50 | g | ||
|
|
Resazurin | 0.50 | mg | ||
|
|
Mineral solution | 0.20 | ml | ||
|
|
Distilled water | 900.00 | ml | ||
|
|
Xylose
(w/v, 10%) |
100.00 | ml | ||
| 1 Mix components thoroughly and adjust pH to 9.0. Distribute the medium into culture vessels (e.g., 9.0 ml in Balch tubes), replace the gas phase with N2, seal with butyl rubber stoppers, and autoclave. After cooling, aseptically and anaerobically add per 900 ml the following solution (autoclaved under a N2 atmosphere): | |||||
Mineral solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
MgCl2 x 6 H2O | 25.000 | g |
|
|
CaCl2 x 2 H2O | 37.500 | g |
|
|
FeSO4 x 7 H2O | 0.312 | g |
|
|
Distilled water | 1000.000 | ml |