1809
1809 .
DC-8A Medium
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
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Medium for Insulambacter thermoxylanivorax DSM 111723
This strain uses the standard medium without modifications.
Growth conditions: anaerobic
55 °C
Main sol. 1809 #
ml
Equipment needed: Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| K2HPO4 | 2.90 | g | 2.9 | 16.65 | |
| Urea | 4.20 | g | 4.2 | 69.935 | |
| Yeast extract | 2.00 | g | 2 | - | |
| CaCl2 x 2 H2O | 0.01 | g | 0.01 | 0.068 | |
| Na2CO3 | 1.00 | g | 1 | 9.435 | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.5 | 2.847 | |
| Resazurin | 0.50 | mg | 5.0e-4 | 0.002 | |
| Mineral solution | 0.20 | ml | - | - | |
| Distilled water | 900.00 | ml | - | - | |
|
Xylose
(w/v, 10%) |
100.00 | ml | 10 | 66.609 | |
| 1 Mix components thoroughly and adjust pH to 9.0. Distribute the medium into culture vessels (e.g., 9.0 ml in Balch tubes), replace the gas phase with N2, seal with butyl rubber stoppers, and autoclave. After cooling, aseptically and anaerobically add per 900 ml the following solution (autoclaved under a N2 atmosphere): | |||||
Solution occurs in 3
other media (first defined in Medium J1274).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| MgCl2 x 6 H2O | 25.000 | g | 25 | 122.969 |
| CaCl2 x 2 H2O | 37.500 | g | 37.5 | 255.076 |
| FeSO4 x 7 H2O | 0.312 | g | 0.312 | 1.122 |
| Distilled water | 1000.000 | ml | - | - |
Metadata
| Last modified: | 27.03.23 |
| Source: | MediaDive: DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 9.0 |
| Equipment needed: | Anaerobic workbench, Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Insulambacter thermoxylanivorax DSM 111723
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Xylose | 10 | 66.609 |
| Urea | 4.2 | 69.935 |
| K2HPO4 | 2.9 | 16.65 |
| Yeast extract | 2 | - |
| Na2CO3 | 1 | 9.435 |
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Cultivation metadata from 1 strains
| phyla | 1 | 1 strain |