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DSMZ

DSMZ 1795 . PAS (Page’s amoeba saline)

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main Solution 1795 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
PAS Stock Solution 1 5 ml - -
PAS Stock Solution 2 5 ml - -
1 Make up to 1000 ml with MilliQ water.
2 Autoclave at 121 °C for 20 min.

PAS Stock Solution 1 #

500
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 12.0 g 24 410.678
MgSO4 x 7 H2O 0.4 g 0.8 3.246
CaCl2 x 2 H2O 0.4 g 0.8 5.442

PAS Stock Solution 2 #

500
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4
(anhydrous) 		14.2 g 28.4 200.058
KH2PO4
(anhydrous) 		13.6 g 27.2 199.875

Metadata

Last modified: 09.06.26
Source: MediaDive: DSMZ
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Autoclave
Reference: Caspers, H. and F. Page (1976).
Description:

Medium to culture Protozoa
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 0.142 1
KH2PO4 0.136 0.999
NaCl 0.12 2.053
CaCl2 x 2 H2O 0.004 0.027
MgSO4 x 7 H2O 0.004 0.016
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