1778a .
ANB aerobic
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Equipment needed:
Autoclave
Final pH:
n.d.
Description:
used for aerobic moderately haloalkalilphilic Planctomycetota strain AB-hyl4 (DSM 117794)
Select a strain (optional):
Main sol. 1778a #
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2CO3 | 15 | g/l | ||
|
|
NaHCO3 | 20 | g/l | ||
|
|
NaCl | 3 | g/l | ||
|
|
K2HPO4 | 1 | g/l | ||
|
|
Trace element solution | 1 | ml | ||
| 1 mix compounds. | |||||
| 2 check pH (should be 10) | |||||
| 3 Fill 100 mL each into 200 mL serum-flasks. | |||||
| 4 Autoclave at 121 °C for 20 min. | |||||
| 5 After autoclaving, add the following sterile solutions: | |||||
|
|
MgSO4
(1M in H2O) |
1 | ml/l | ||
|
|
NH4Cl
(2M in H2O) |
2 | ml/l | ||
|
|
Vitamine mixture | 1 | ml/l | ||
|
|
Melezitose
(10% in H2O) |
10 | ml/l | ||
|
|
Yeast extract
(1% solution in water) |
2 | ml/l | ||
| 6 Mix well and aliquot into the desired volumes and flasks or tubes (e.g., 20 ml in 100 ml flasks). | |||||
Trace element solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
EDTA | 5.00 | g | ||
|
|
FeSO4 x 7 H2O | 2.00 | g | ||
|
|
ZnSO4 | 0.10 | g | ||
|
|
MnCl2 | 0.03 | g | ||
|
|
H3BO3 | 0.30 | g | ||
|
|
CoCl2 x 6 H2O | 0.20 | g | ||
|
|
CuCl2 x 5 H2O | 0.01 | g | ||
|
|
NiCl2 x 6 H2O | 0.02 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.02 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 3.0-4.0 with HCl. | |||||
| 2 Autoclave at 121 °C for 15 min. | |||||
| 3 Note: autoclave in closed bottles | |||||
Vitamine mixture #
400
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Acidic thiamine / Ca pantothenate solution | 100 | ml | ||
|
|
Basic vitamin solution | 100 | ml | ||
|
|
Neutral folic acid / riboflavin solution | 100 | ml | ||
|
|
Neutral B12 solution 0.005 g/L | 100 | ml | ||
| 1 Sterilize by filtration. | |||||
| 2 Mix the four solutions in the ratio 1:1:1:1. | |||||
Acidic thiamine / Ca pantothenate solution #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Thiamine | 5 | mg |
|
|
Calcium pantothenate | 5 | mg |
|
|
Distilled water
(acidified to pH 3.0 with HCl) |
100 | ml |
Basic vitamin solution #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2 | mg |
|
|
PABA
(p-aminobenzoic acid) |
5 | mg |
|
|
Nicotinic acid | 5 | mg |
|
|
Pyridoxine | 10 | mg |
|
|
NaOH
(0.1N) |
100 | ml |
Neutral folic acid / riboflavin solution #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Folic acid | 2 | mg |
|
|
Riboflavin | 5 | mg |
|
|
Distilled water | 100 | ml |