167 .
SPOROCYTOPHAGA MEDIUM
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* Medium for Sporocytophaga myxococcoides DSM 11118
It is recommended to use the following modified medium for the reactivation of freeze-dried samples: Prepare agar plates of medium 167 by supplementing medium with 1.5% (w/v) agar. Place 4 pieces of sterile cellulose (not nitrocellulose!) filter paper on each agar plate. Suspend freeze-dried content of the inner vial of one ampoule with 0.5 ml of liquid medium 167 and inoculate each piece of the filter paper with 1 large drop of the cell suspension. Alternatively, the strain also grows with 0.1% glucose or 0.1% cellobiose as carbon source.
Growth conditions: 3-7 days
aerobic
25 °C
Equipment needed:
Filter
Final pH:
7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaNO3 | 2.00 | g | ||
|
|
MgSO4 x 7 H2O | 1.00 | g | ||
|
|
KCl | 0.50 | g | ||
|
|
FeSO4 x 7 H2O | 6.00 | mg | ||
|
|
KH2PO4 | 0.14 | g | ||
|
|
K2HPO4 | 1.20 | g | ||
|
|
Yeast extract | 0.02 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
|
|
Agar
optional |
15.00 | g/l | ||
|
|
D-Glucose
(optional) |
1.00 | g/l | ||
|
|
Cellobiose
(optional) |
1.00 | g/l | ||
| 1 Adjust pH to 7.2. | |||||
| 2 Place a strip of sterile cellulose filter paper onto an agar plate. Alternatively, place a strip of sterile cellulose filter paper into a culture tube containing 5 ml of medium, thereby leaving 1 - 2 cm of the strip outside of the medium. Inoculate the strain onto the cellulose filter paper. The strain will grow on the cellulose paper only. | |||||