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DSMZ

DSMZ 1668 . MDM (modified dehority medium)

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Medium for Hallerella succinigenes DSM 104698

* Modification: For 10 ml in hungate tube, add: Vitamin solution 5-10 µl, Rumen fluid 500 µl, and Cellobiose (10%) 200 µl

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 1-2 days anaerobic 37 °C

Main sol. 1668 #

ml
Equipment needed: Hungate tubes Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.5 g 1 -
Tryptone 0.5 g 1 14.069
Stock A: Salt solution 2x (50 ml stock per 100 ml medium) 250.0 ml - -
Stock B: Salt solution 200x (0,5 ml stock per 100 ml medium) 2.5 ml - -
Distilled water 250.0 ml - -
L-Cysteine HCl 1.0 g/l 1 6.344
1 To make medium anoxic boil it, cool under N2CO2 gas atmosphere, add 1 g/l L-cysteine hydrochloride and adjust pH to 6.8. Finally gas over with N2 . Dispense under N2CO2 into suitable vessels for anaerobic growth (serum bottles or Hungate tubes sealed with rubber stoppers) and sterilize by autoclaving.

Vitamin solution #

1000
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine-HCl x 2 H2O 5.0 mg 0.005 0.013
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
D-Calcium pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -
1 Filter sterilize and make anoxic with N2.

Cellobiose (10%) #

100
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Distilled water 100 ml - -
Cellobiose 10 g 100 292.141
1 Mix 10 g cellobiose with 100 ml distilled water and filter sterilize, make anoxic with N2.

CM-Mix (only for DSM 104697) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
D-Glucose 4 g 4 22.203
Cellobiose 1 g 1 2.921
Maltose 1 g 1 2.921
Starch
(soluble) 		1 g 1 -
Distilled water 1000 ml - -
1 Make anoxic with N2.

Vitamin K1 solution (only for DSM 104697) #

20
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Ethanol
(95 %) 		20.0 ml 19 412.416
Vitamin K1 0.1 ml - -
1 Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Make anoxic with N2. Store refrigerated in a brown bottle.

Stock A: Salt solution 2x (50 ml stock per 100 ml medium) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 6804 mg 6.804 39.064
NaHCO3 6721 mg 6.721 80.005
NaCl 4675 mg 4.675 79.997
NH4Cl 1605 mg 1.605 30.006
CaCl2 89 mg 0.089 0.802
MgCl2 95 mg 0.095 0.998
FeSO4 x 7 H2O 28 mg 0.028 0.101
Distilled water 1000 ml - -

Stock B: Salt solution 200x (0,5 ml stock per 100 ml medium) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgCl2 x 2 H2O 971 mg 0.971 7.399
ZnCl2 273 mg 0.273 2.003
CoCl2 104 mg 0.104 0.801
Na2 MoO4 x 2 H2O 48 mg 0.048 0.198
Na2SeO3 35 mg 0.035 0.202
NiCl2 x 6 H2O 237 mg 0.237 1.829
Na2WO4 x 2 H2O 66 mg 0.066 0.2
Distilled water 1000 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Anaerobic workbench, Filter, Hungate tubes
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Associated strains

All strains for this medium: 4
Strains with modifications: 4
Hallerella succinigenes DSM 104698

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Tryptone 1 14.069
L-Cysteine HCl 1 6.344
Yeast extract 1 -
K2HPO4 0.014 0.078
NaHCO3 0.013 0.16
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Cultivation metadata from 4 strains BacDive

phyla 1 4 strains
oxygen anaerobe 1 strain
oxygen obligate anaerobe 1 strain
temperature mesophilic 4 strains