1663
1663 .
BSM medium
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Desulfuribacillus stibiiarsenatis DSM 28709
This strain uses the standard medium without modifications.
Growth conditions: 35 °C
Main sol. 1663 #
ml
Equipment needed: Gassing station
Autoclave
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 3.62 | g | 3.62 | 61.944 | |
| K2HPO4 | 0.26 | g | 0.26 | 1.493 | |
| (NH4)Cl | 0.08 | g | 0.08 | 1.496 | |
| NaNO3 | 0.85 | g | 0.85 | 10.001 | |
| Na2SO4 | 0.05 | g | 0.05 | 0.352 | |
| Trace element solution | 1.00 | ml | - | - | |
| Wolin's vitamin solution | 10.00 | ml | - | - | |
| Na-DL-lactate | 1.12 | g | 1.12 | 9.995 | |
| Yeast extract | 0.20 | g | 0.2 | - | |
| Distilled water | 936.50 | ml | - | - | |
|
NaHCO3
(8%) |
52.50 | ml | 4.2 | 49.996 | |
| 1 Dissolve ingredients (except vitamins and trace elements) in distilled water; boil for 1- 2 minutes and cool down to room temperature under 100% N2 gas, dispense in culture vessels and autoclave. After sterilization add NaHCO3, vitamins, and trace elements from sterile anoxic stock solutions prepared under 100% N2. Adjust pH to 8.2 and add sodium sulfide (Na2S) from a sterile anoxic solution to achieve a final concentration of 100 µM prior inoculation. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%; 7.7 M) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| MgCl2 x 6 H2O | 3.0 | g | 3 | 14.756 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.68 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml. | |||||
Solution occurs in 53
other media (first defined in Medium 141).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium D-(+)-pantothenate | 5.0 | mg | 0.005 | 0.021 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| (DL)-alpha-Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 27.04.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 8.2 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Desulfuribacillus stibiiarsenatis DSM 28709
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaHCO3 | 4.2 | 49.996 |
| NaCl | 3.62 | 61.944 |
| Na-DL-lactate | 1.12 | 9.995 |
| NaNO3 | 0.85 | 10.001 |
| K2HPO4 | 0.26 | 1.493 |
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