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DSMZ

DSMZ 1630a . NITROSOPUMILUS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Nitrosopumilus adriaticus DSM 106890

This strain uses the standard medium without modifications.

Growth conditions: more than 4 weeks aerobic 28 °C

Main sol. 1630a #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 26.00 g 26 444.901
MgSO4 x 7 H2O 5.00 g 5 20.286
MgCl2 x 6 H2O 5.00 g 5 24.594
CaCl2 x 2 H2O 1.50 g 1.5 10.203
KBr 0.10 g 0.1 0.84
KH2PO4
(0.1% w/v) 		2.00 ml 0.002 0.015
HEPES buffer solution 10.00 ml - -
NH4Cl 0.05 g 0.05 0.935
Trace element solution SL-11 1.00 ml - -
Na-pyruvate 0.05 g 0.05 0.454
NaHCO3 0.25 g 0.25 2.976
Wolin's vitamin solution 10.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except trace elements, HEPES buffer, ammonium chloride, pyruvate, bicarbonate and vitamins), dispense medium into Erlenmeyr flasks with screw caps (e.g., 20 ml medium per 100 ml flask) and autoclave. Add HEPES buffer and ammonium chloride from stock solutions sterilized by autoclaving. Trace elements, pyruvate, bicarbonate and vitamins are added from stock solutions sterilized by filtration. Adjust pH of the complete medium to 7.2 using sterile solutions of bicarbonate (5% w/v) or 1 N HCl.
2 Note: Use at least 5% (v/v) inoculum and incubate in the dark without shaking.

HEPES buffer solution #

1000
Solution occurs in 3 other media (first defined in Medium 1630).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH
(pellets) 		24.0 g 24 600.045
HEPES
(free acid) 		238.4 g 238.4 1000.42
Distilled water 1000.0 ml - -
1 Fist dissolve NaOH in ca 600 ml water, then add HEPES, adjust pH to 7.5 and fill up to 1000 ml volume.

Trace element solution SL-11 #

1000
Solution occurs in 22 other media (first defined in Medium 722).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA x 2 H2O 5.2 g 5.2 15.467
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 1000.0 ml - -
1 Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.

Wolin's vitamin solution #

1000
Solution occurs in 53 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 0.021
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 14.04.22
Source: DSMZ
Medium type: Defined medium
Final pH: 7.2
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 3
Nitrosopumilus adriaticus DSM 106890

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 26 444.901
MgCl2 x 6 H2O 5 24.594
MgSO4 x 7 H2O 5 20.286
HEPES 2.384 10.004
CaCl2 x 2 H2O 1.5 10.203
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Cultivation metadata from 3 strains BacDive

pH 6 - 8 2 strains
phyla 2 3 strains
oxygen aerobe 2 strains
temperature mesophilic 2 strains