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HEPES buffer solution

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Internal Solution-ID: S3383

This solution is used in 4 media and was first defined in medium 1630.

Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH
(pellets) 		24.0 g 24 600.045
HEPES
(free acid) 		238.4 g 238.4 1000.42
Distilled water 1000.0 ml - -
1 Fist dissolve NaOH in ca 600 ml water, then add HEPES, adjust pH to 7.5 and fill up to 1000 ml volume.
1630c modified Nitrosospaera medium
1630a NITROSOPUMILUS MEDIUM
1630 NITROSOSPHAERA MEDIUM
1630e Synthetic Crenarchaeota Medium (SCM)