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DSMZ

DSMZ 1600 . FRANCISELLA PERSICA MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Francisella persica DSM 101678

* Modification: Plus 5% CO2

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 3-7 days 34 °C

Main sol. 1600 #

ml
Equipment needed: Filter
1 2x concentrated stock:
Compound Amount Unit Conc. [g/L] Conc. [mM]
L-15 powder
(Leibovitz medium) 		1.000 pack - -
DL-aspartic acid 0.299 g 0.835 6.275
L-Glutamic acid 0.500 g 1.397 9.493
L-Glutamine
(200 mM) 		10.000 ml 0.292 2
L-Proline 0.300 g 0.838 7.279
alpha-ketoglutaric acid 0.299 g 0.835 5.717
D-Glucose 2.239 g 6.254 34.715
Stock Solution D (10 ml) 1.000 ml - -
Vitamin Stock (10 ml) 1.000 ml - -
Demineralized water 346.000 ml - -
2 Adjust pH to 6.8 with NaOH.
3 When ready to use add:
4 Aliquot 17.3ml into 50ml tubes and store at -20°C.
5 Fill up to 346ml with demineralized water and filter sterilize through 0.2µm filter
Demineralized water
(sterile ) 		25.000 ml - -
Tryptose-phosphate
(broth) 		5.000 ml - -
FBS
(heat inactivated) 		2.500 ml - -
cholesterol lipid concentrate
(250x, ThermoFisher Scientific) 		200.000 µl - -
6 Filter sterilize each tube through 0.2µm filter.

Stock Solution A (10 ml) #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.20 g 20 69.551
MnSO4 x 1 H2O 0.16 g 16 105.96
CuSO4 x 5 H2O 0.02 g 2 8.01
CoCl2 x 6 H2O 0.02 g 2 15.404

Stock Solution B (10 ml) #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2 MoO4 x 2 H2O 0.02 g 2 8.266

Stock Solution C (10 ml) #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SeO3 0.02 g 2 11.565

Stock Solution D (10 ml) #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Glutathione
(reduced) 		1.00 g 100 325.394
Ascorbic acid 1.00 g 100 567.795
FeSO4 x 7 H2O 0.05 g 5 17.985
Stock Solution A (10 ml) 0.10 ml - -
Stock Solution B (10 ml) 0.10 ml - -
Stock Solution C (10 ml) 0.10 ml - -

Vitamin Stock (10 ml) #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Para-aminobenzoic acid 0.10 g 10 72.918
Vitamin B12 0.05 g 5 3.689
D-Biotin 0.01 g 1 4.093

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Filter
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Gas composition

  • 5 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1
Strains with modifications: 1
Francisella persica DSM 101678

  5% CO2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
D-Glucose 6.254 34.715
L-Glutamic acid 1.397 9.493
L-Proline 0.838 7.279
DL-aspartic acid 0.835 6.275
alpha-ketoglutaric acid 0.835 5.717
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain