Main sol. 1600
Internal Solution-ID: S3336
Equipment needed: Filter
| 1 2x concentrated stock: | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
L-15 powder
(Leibovitz medium) |
1.000 | pack | - | - | |
| DL-aspartic acid | 0.299 | g | 0.835 | 6.275 | |
| L-Glutamic acid | 0.500 | g | 1.397 | 9.493 | |
|
L-Glutamine
(200 mM) |
10.000 | ml | 0.292 | 2 | |
| L-Proline | 0.300 | g | 0.838 | 7.279 | |
| alpha-ketoglutaric acid | 0.299 | g | 0.835 | 5.717 | |
| D-Glucose | 2.239 | g | 6.254 | 34.715 | |
| Stock Solution D (10 ml) | 1.000 | ml | - | - | |
| Vitamin Stock (10 ml) | 1.000 | ml | - | - | |
| Demineralized water | 346.000 | ml | - | - | |
| 2 Adjust pH to 6.8 with NaOH. | |||||
| 3 When ready to use add: | |||||
| 4 Aliquot 17.3ml into 50ml tubes and store at -20°C. | |||||
| 5 Fill up to 346ml with demineralized water and filter sterilize through 0.2µm filter | |||||
|
Demineralized water
(sterile ) |
25.000 | ml | - | - | |
|
Tryptose-phosphate
(broth) |
5.000 | ml | - | - | |
|
FBS
(heat inactivated) |
2.500 | ml | - | - | |
|
cholesterol lipid concentrate
(250x, ThermoFisher Scientific) |
200.000 | µl | - | - | |
| 6 Filter sterilize each tube through 0.2µm filter. | |||||
Media with this solution
| 1600 | FRANCISELLA PERSICA MEDIUM |