DSMZ 1593 . Cyanobacteria Medium BG11+

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

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Medium for Gomphosphaeria aponina DSM 107014

This strain uses the standard medium without modifications.


Growth conditions: 17 °C
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Mg(SO4) x 7 H2O
(7.5 g/l stock solution)
10 ml - -
CaCl2 x 2 H2O
(3.6 g/l stock solution)
10 ml - -
Na3-citrate x 2 H2O
(0.6 g/l stock solution)
10 ml - -
Na-EDTA x 2 H2O
(0.1 g/l stock solution)
10 ml - -
Trace metal mix 1 ml - -
1 Make up to 1000 ml with MilliQ water.
2 Autoclave at 121 °C for 20 min.
3 After cooling, add the following filter sterilized components.
NaNO3
(300 g/l stock solution)
5 ml 1.5 17.648
BG11-Mix 2 ml - -
Vitamin B12
(0.02 g/l stock solution)
1 ml - -
Solution occurs in 2 other media (first defined in Medium 1592).
Compound Amount Unit Conc. [g/L] Conc. [mM]
H3BO3 2.90 g 2.9 46.895
MnCl2 x 4 H2O 1.81 g 1.81 9.146
ZnSO4 x 7 H2O 0.22 g 0.22 0.765
Na2MoO4 x 2 H2O 0.39 g 0.39 1.612
CuSO4 x 5 H2O
(0.8 g/100 ml stock solution)
10.00 ml - -
Co(NO3)2 x 6 H2O
(0.5 g/100 ml stock solution)
10.00 ml - -
1 Make up to 1000 ml with MilliQ water.
Solution occurs in 3 other media (first defined in Medium 1592).
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 x 3 H2O 20 g 20 114.826
Na2CO3 10 g 10 94.35
Fe(NH4)citrate 3 g 3 -
1 Make up to 1000 ml with MilliQ water.

Metadata

Last modified: 09.11.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 46
Strains with modifications: 33
Gomphosphaeria aponina DSM 107014

No modifications necessary for this strain.

Light

33 strains need light for growth. Select a strain for more details.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaNO3 1.5 17.648
K2HPO4 x 3 H2O 0.04 0.23
Na2CO3 0.02 0.189
Fe(NH4)citrate 0.006 -
H3BO3 0.003 0.047
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Cultivation metadata from 46 strains BacDive

phyla 1 47 strains
temperature psychrophilic 40 strains