1593a
1593a .
Cyanobacteria Medium BG11+ (TES buffered)
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1593a #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| TES | 2.3 | g | 2.3 | 10.033 | |
|
NaNO3
(300 g/l stock solution) |
5.0 | ml | - | - | |
|
Mg(SO4) x 7 H2O
(7.5 g/l stock solution) |
10.0 | ml | - | - | |
|
CaCl2 x 2 H2O
(3.6 g/l stock solution) |
10.0 | ml | - | - | |
|
Na3-citrate x 2 H2O
(0.6 g/l stock solution) |
10.0 | ml | - | - | |
|
Na-EDTA x 2 H2O
(0.1 g/l stock solution) |
10.0 | ml | - | - | |
|
K2HPO4 x 3 H2O
(20 g/l stock solution) |
2.0 | ml | - | - | |
|
Na2CO3
(10 g/l stock solution) |
2.0 | ml | - | - | |
|
Fe(NH4)citrate
(6.0 g/l stock solution) |
1.0 | ml | - | - | |
| Trace metal mix | 1.0 | ml | - | - | |
| 1 Adjust pH to 8.0. | |||||
| 2 Make up to 1000 ml with MilliQ water. | |||||
| 3 Autoclave at 121 °C for 20 min. | |||||
| 4 After Cooling, add the following filter sterilized components: | |||||
|
Vitamin B12
(0.02 g/l stock solution) |
1.0 | ml | - | - | |
Solution occurs in 6
other media (first defined in Medium 1592).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| H3BO3 | 2.86 | g | 2.9 | 46.895 | |
| MnCl2 x 4 H2O | 1.81 | g | 1.81 | 9.146 | |
| ZnSO4 x 7 H2O | 0.22 | g | 0.22 | 0.765 | |
| Na2MoO4 x 2 H2O | 0.39 | g | 0.39 | 1.612 | |
|
CuSO4 x 5 H2O
(8.0 g/l stock solution) |
10.00 | ml | - | - | |
|
Co(NO3)2 x 6 H2O
(5.0 g/l stock solution) |
10.00 | ml | - | - | |
| 1 Make up to 1000 ml with MilliQ water. | |||||
Metadata
| Last modified: | 19.03.25 |
| Source: | MediaDive: DSMZ |
| Medium type: | Complex medium |
| Final pH: | 8.0 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 2 |