150 .
ACIDIANUS BRIERLEYI MEDIUM
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Selected strain: Acidianus brierleyi DSM 1651
This strain uses the standard medium without modifications.
Growth conditions: 70 °C
Equipment needed:
Autoclave
Water bath
Final pH:
1.5 - 2.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
(NH4)2SO4 | 3.00 | g | ||
|
|
K2HPO4 x 3 H2O | 0.50 | g | ||
|
|
MgSO4 x 7 H2O | 0.50 | g | ||
|
|
KCl | 0.10 | g | ||
|
|
Ca(NO3)2 x 4 H2O | 0.02 | g | ||
|
|
Yeast extract | 0.20 | g | ||
|
|
Sulfur
(powdered) |
10.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve ingredients except yeast extract and sulfur. Adjust pH with 6 N H2SO4 to 1.5 - 2.5. Yeast extract (10% w/v in distilled water) is autoclaved separately. For sterilization of the sulfur place it in screw-capped tubes or bottles, wet with a few drops of water and heat for 3 hours to 90 - 100°C in a water bath on each of three successive days. Before use, aseptically layer the sulfur onto the surface of the autoclaved liquid basal medium. | |||||