1504 .
CM MEDIUM
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Selected strain: Actinopolyspora sp. DSM 46669
This strain uses the standard medium without modifications.
Growth conditions: 28 °C
Equipment needed:
Final pH:
7.4
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Peptone | 7.5 | g | ||
|
|
Yeast extract | 10.0 | g | ||
|
|
MgSO4 x 7 H2O | 10.0 | g | ||
|
|
Na3-citrate | 3.0 | g | ||
|
|
KCl | 2.0 | g | ||
|
|
NaCl | 150.0 | g | ||
|
|
FeSO4 x 7 H2O | 1.0 | ml | ||
|
|
Agar | 20.0 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 pH 7.4 (NaOH) | |||||
| 2 Prepare 4.98% FeSO4 x 7 H2O (= 10 g Fe in 1l 0.001 M HCl); add 1 ml per liter medium. | |||||