# 1504: CM MEDIUM


## Main sol. 1504

7.5	g	Peptone	
10	g	Yeast extract	
10	g	MgSO4 x 7 H2O	
3	g	Na3-citrate	
2	g	KCl	
150	g	NaCl	
1	ml	FeSO4 x 7 H2O	
20	g	Agar	
1000	ml	Distilled water	

1. pH 7.4 (NaOH)
2. Prepare 4.98% FeSO4 x 7 H2O (= 10 g Fe in 1l 0.001 M HCl); add 1 ml per liter medium.