1495 .
AQUASPIRILLUM MEDIUM II
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Selected strain: Giesbergeria voronezhensis DSM 12828
This strain uses the standard medium without modifications.
Growth conditions: 28 °C
Equipment needed:
Autoclave
Filter
Final pH:
7.4 - 7.6
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Peptone
(BD) |
2.00 | g | ||
|
|
Succinic acid | 1.00 | g | ||
|
|
(NH4)2SO4 | 1.00 | g | ||
|
|
MgSO4 x 7 H2O | 1.00 | g | ||
|
|
CaCl2 x 2 H2O | 0.03 | g | ||
|
|
Vitamin solution | 1.00 | ml | ||
|
|
Trace elements solution (Pfennig & Lippert, 1966) | 1.00 | ml | ||
|
|
Distilled water | 1000.00 | ml | ||
|
|
Vitamin solution | 1.00 | ml | ||
|
|
Agar , for solid medium | 15.00 | g | ||
| 1 Adjust pH to 7.4-7.6 Autoclave and add 1.0 ml/l vitamin solution from a filter sterilised stock solution (see below). The medium may be solidified by adding 15.0 g/l agar. | |||||
Trace elements solution (Pfennig & Lippert, 1966) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
EDTA | 5.00 | g | ||
|
|
FeSO4 x 6 H2O | 2.20 | g | ||
|
|
ZnSO4 x 7 H2O | 0.10 | g | ||
|
|
MnCl2 x 4 H2O | 0.03 | g | ||
|
|
H3BO3 | 0.03 | g | ||
|
|
CoCl2 x 6 H2O | 0.20 | g | ||
|
|
CuCl2 x 2 H2O | 0.03 | g | ||
|
|
NiCl2 x 6 H2O | 0.03 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.03 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 pH 3.0-4.0 | |||||
Vitamin solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Biotin | 200 | mg | ||
|
|
Folic acid | 20 | mg | ||
|
|
Pyridoxine hydrochloride | 100 | mg | ||
|
|
Thiamine HCl | 50 | mg | ||
|
|
Riboflavin | 100 | mg | ||
|
|
Nicotinic acid | 50 | mg | ||
|
|
DL-Pantothenic acid | 50 | mg | ||
|
|
Vitamin B12 | 1 | mg | ||
|
|
Para-aminobenzoic acid | 50 | mg | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Filter sterilise. | |||||