This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaHCO3 | 0.50 | g | 0.5 | 5.952 | |
NaCl | 0.50 | g | 0.5 | 8.556 | |
KH2PO4 | 0.40 | g | 0.4 | 2.939 | |
(NH4)2SO4 | 0.20 | g | 0.2 | 1.514 | |
MgSO4 x 6 H2O | 0.10 | g | 0.1 | 0.831 | |
CaCl2 | 0.01 | g | 0.01 | 0.09 | |
Li2B4O7 | 0.01 | g | 0.01 | - | |
FeEDTA solution | 1.00 | ml | - | - | |
Trace element solution | 1.00 | ml | - | - | |
Distilled water | 1000.00 | ml | - | - | |
1 Do not adjust the pH. | |||||
2 For plates add 8g/l Gelrite or 15g Phytagel and 1g/L MgCl. A carbon source is not required because the organism degrades the polysaccharides in Gelrite or Phytagel | |||||
3 For liquid medium use 0.8-2.0g/l Gelrite as carbon source (the medium remains liquid). Alternatively mannose (1g/l) may be used instead of Gelrite in liquid medium. Do not add mannose to solid media, which will inhibit growth. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
FeSO4 x 7 H2O | 1.54 | g | 1.54 | 5.539 |
Na-EDTA | 2.06 | g | 2.06 | 6.127 |
Distilled water | 1000.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 1.500 | g | 1.5 | 7.848 | |
Fe(NH4)2(SO4)2 x 6 H2O | 0.200 | g | 0.2 | - | |
Na2SeO4 x 10 H2O | 0.440 | g | 0.44 | - | |
CoCl2 x 6 H2O | 0.180 | g | 0.18 | 1.386 | |
MnSO4 x H2O | 0.120 | g | 0.12 | 0.71 | |
Na2MoO4 | 0.160 | g | 0.16 | 0.777 | |
Na2WO4 | 0.100 | g | 0.1 | 0.34 | |
ZnSO4 x 7 H2O | 0.540 | g | 0.54 | 1.878 | |
AlCl3 | 0.040 | g | 0.04 | 0.3 | |
NiCl2 x 6 H2O | 0.025 | g | 0.025 | 0.193 | |
H3BO3 | 0.110 | g | 0.11 | 1.779 | |
CuSO4 | 0.210 | g | 0.21 | 1.316 | |
MnCl2 | 0.190 | g | 0.19 | 1.51 | |
Distilled water | 1000.000 | ml | - | - | |
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. | |||||
2 Final pH 7.0 (with KOH). |
Last modified: | 08.09.22 |
Source: | DSMZ |
Synonyms: | . VERRUCOMICROBIA MEDIUM |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | 7.0 |
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All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 0.5 | 8.556 |
NaHCO3 | 0.5 | 5.952 |
KH2PO4 | 0.4 | 2.939 |
(NH4)2SO4 | 0.2 | 1.514 |
MgSO4 x 6 H2O | 0.1 | 0.831 |
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