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DSMZ

DSMZ 1487 . MAGNETOVIBRIO MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Medium for Magnetovibrio blakemorei DSM 18854

This strain uses the standard medium without modifications.

Growth conditions: 3-7 days anaerobic 28 °C

Main sol. 1487 #

ml
Equipment needed: Autoclave Hungate tubes Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 16.50 g 16.321 279.269
MgCl2 x 6 H2O 3.50 g 3.462 17.028
Na2SO4 2.75 g 2.72 19.15
KCl 0.50 g 0.495 6.634
NaNO3 0.50 g 0.495 5.819
CaCl2 x 2 H2O 0.40 g 0.396 2.691
NH4Cl 0.25 g 0.247 4.623
Trace element solution 10.00 ml - -
Na-acetate 0.25 g 0.247 3.014
Disodium succinate 0.50 g 0.495 3.052
Casamino acids
(BD Bacto) 		0.50 g 0.495 -
Sodium resazurin
(0.1% w/v) 		0.50 ml 4.9e-4 0.002
K2HPO4 0.15 g 0.148 0.852
Na2CO3 1.50 g 1.484 13.999
Wolin's vitamin solution (10x) 1.00 ml - -
L-Cysteine HCl x H2O 0.25 g 0.247 1.408
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except hydrogenphosphate, carbonate, vitamins and cysteine), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add hydrogenphosphate, vitamins and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. The vitamin solution should be sterilized by filtration. Adjust pH of the complete medium to 7.0 - 7.2, if necessary.

Trace element solution #

1000
Solution occurs in 14 other media (first defined in Medium 318).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA) 		12.80 g 12.8 66.967
FeCl2 x 4 H2O 1.00 g 1 5.03
MnCl2 x 4 H2O 0.10 g 0.1 0.505
CoCl2 x 6 H2O 0.03 g 0.03 0.231
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnCl2 0.10 g 0.1 0.734
CuCl2 0.02 g 0.02 0.149
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
NiCl2 x 6 H2O 0.10 g 0.1 0.772
NaCl 1.00 g 1 17.112
Na2SeO3 x 5 H2O 0.03 g 0.03 0.114
Na2WO4 x 2 H2O 0.04 g 0.04 0.121
Distilled water 1000.00 ml - -
1 First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. Finally adjust pH to 6.5 with KOH and make up to 1000.00 ml.

Wolin's vitamin solution (10x) #

1000
Solution occurs in 305 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 04.05.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0 - 7.2
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1
Magnetovibrio blakemorei DSM 18854

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 3.462 17.028
Na2SO4 2.72 19.15
Na2CO3 1.484 13.999
KCl 0.495 6.634
NaNO3 0.495 5.819
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
oxygen microaerophile 1 strain
temperature mesophilic 1 strain