1382 .
MP MEDIUM
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Equipment needed:
Final pH:
5.0 - 6.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 0.10 | g | ||
|
|
CaCl2 x 2 H2O | 0.02 | g | ||
|
|
NH4Cl | 0.20 | g | ||
|
|
Yeast extract | 0.10 | g | ||
|
|
Glucose | 0.50 | g | ||
|
|
Trace element solution (SLA) | 1.00 | ml | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 5.0-6.5 | |||||
| 2 Serum flasks are filled with Medium by 5/6 of the total volume. | |||||
| 3 Sterilize at 121°C for 15 minutes. | |||||
Trace element solution (SLA) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
FeCl2 x 4 H2O | 1.8 | g | ||
|
|
CoCl2 x 6 H2O | 250.0 | mg | ||
|
|
NiCl2 x 6 H2O | 10.0 | mg | ||
|
|
CuCl2 x 2 H2O | 10.0 | mg | ||
|
|
MnCl2 x 4 H2O | 70.0 | mg | ||
|
|
ZnCl2 | 100.0 | mg | ||
|
|
H3BO3 | 500.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 30.0 | mg | ||
|
|
Na2SeO3 x 5 H2O | 10.0 | mg | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Adjust pH to 2.0 - 3.0. | |||||