1372 .
SUCCINATE MINIMAL SALTS MEDIUM (SMS)
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Selected strain: Rhodomicrobium vannielii DSM 23296
This strain uses the standard medium without modifications.
Growth conditions: 3-7 days
28 °C
Equipment needed:
Autoclave
Filter
Final pH:
6.8
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
EDTA | 0.010 | g | ||
|
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KH2PO4 | 0.600 | g | ||
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K2HPO4 | 0.900 | g | ||
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NH4Cl | 1.000 | g | ||
|
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MgSO4 x 7 H2O | 0.200 | g | ||
|
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CaCl2 x 6 H2O | 0.075 | g | ||
|
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Sodium succinate | 2.200 | g | ||
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Yeast extract | 0.100 | g | ||
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Trace elements solution | 2.000 | ml | ||
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Vitamin solution | 2.000 | ml | ||
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Distilled water | 1000.000 | ml | ||
| 1 Adjust pH to 6.8. | |||||
| 2 Prepare the medium without the vitamin solution. | |||||
| 3 Autoclave and add the vitamin solution from a filter-sterilised stock solution. | |||||
| 4 Incubate strains in liquid culture with air headspace in closed vessels. | |||||
Trace elements solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
FeSO4 x 6 H2O | 0.3 | g |
|
|
ZnSO4 x 7 H2O | 5.0 | mg |
|
|
MnCl2 x 4 H2O | 3.0 | mg |
|
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H3BO3 | 2.0 | mg |
|
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CoCl2 x 6 H2O | 5.0 | mg |
|
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CuCl2 x 2 H2O | 1.0 | mg |
|
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NiCl2 x 6 H2O | 2.0 | mg |
|
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Na2MoO4 x 2 H2O | 3.0 | mg |
|
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Distilled water | 1000.0 | ml |
Vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 80 | mg |
|
|
Thiamine-HCl x 2 H2O | 400 | mg |
|
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Nicotinic acid | 400 | mg |
|
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Vitamin B12 | 20 | mg |
|
|
Distilled water | 1000 | ml |