136
136 .
VEILLONELLA MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Veillonella parvula DSM 2007
* Modification: Putrescine is essential!
Growth conditions: 1-2 days
anaerobic
37 °C
pH 7.00
Main sol. 136 #
ml
Equipment needed: Autoclave
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Trypticase
(BBL) |
5.00 | g | 5 | - | |
| Yeast extract | 3.00 | g | 3 | - | |
| Na-(DL)-lactate | 7.50 | g | 7.5 | - | |
| Na-thioglycolate | 0.75 | g | 0.75 | 6.573 | |
| Tween 80 | 1.00 | ml | - | - | |
| Glucose | 1.00 | g | 1 | 5.551 | |
| Putrescine | 3.00 | mg | 0.003 | 0.034 | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 7.5 with solid K2CO3. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.5 |
| Equipment needed: | Autoclave, Hungate tubes |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Na-(DL)-lactate | 7.5 | - |
| Trypticase | 5 | - |
| Yeast extract | 3 | - |
| Glucose | 1 | 5.551 |
| Na-thioglycolate | 0.75 | 6.573 |
| Show more | ||
Cultivation metadata from 12 strains
| phyla | 1 | 11 strains |
| oxygen | anaerobe | 11 strains |
| temperature | mesophilic | 11 strains |