1304
1304 .
SULFURITALEA MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1304 #
ml
Equipment needed: Filter
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MgCl2 x 6 H2O | 0.2 | g | 0.199 | 0.981 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.678 | |
| NaNO3 | 1.7 | g | 1.695 | 19.941 | |
| (NH4)2SO4 | 0.1 | g | 0.1 | 0.755 | |
| KH2PO4 | 0.1 | g | 0.1 | 0.733 | |
| KCl | 0.1 | g | 0.1 | 1.337 | |
| Trace element solution SL-10 | 1.0 | ml | - | - | |
| Selenite-tungstate solution | 1.0 | ml | - | - | |
| Na2S2O3 x 5 H2O | 0.4 | g | 0.399 | 1.607 | |
| NaHCO3 | 5.0 | g | 4.985 | 59.341 | |
| Wolin's vitamin solution | 1.0 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients except thiosulfate, bicarbonate, and vitamins, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to remove dissolved oxygen and to saturate the solution with CO2. Dispense solution under 80% N2 and 20% CO2 gas atmosphere in vials suitable for anaerobic cultures (e.g., Balch-type tubes) to 30% of volume. Close vials with butyl rubber septa and autoclave. Add vitamins from a sterile anoxic stock solution prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. The vitamin solution should be sterilized by filtration. Adjust the pH of the complete medium to 6.7 - 7.0, if necessary. | |||||
| 2 After inoculation, add 1 bar overpressure of sterile 100% H2 gas. | |||||
| 3 Note: Use at least 10% (v/v) as inoculum. | |||||
Solution occurs in 266
other media (first defined in Medium 320).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Solution occurs in 179
other media (first defined in Medium 385).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaOH | 0.5 | g | 0.5 | 12.501 |
| Na2SeO3 x 5 H2O | 3.0 | mg | 0.003 | 0.011 |
| Na2WO4 x 2 H2O | 4.0 | mg | 0.004 | 0.012 |
| Distilled water | 1000.0 | ml | - | - |
Solution occurs in 54
other media (first defined in Medium 141).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium D-(+)-pantothenate | 5.0 | mg | 0.005 | 0.021 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| (DL)-alpha-Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 29.10.24 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 6.7 - 7.0 |
| Equipment needed: | Autoclave, Filter, Gassing station |
| Edit in Medium builder | |
Gas composition
- 50 % H2
- 40 % N2
- 10 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 3 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaHCO3 | 4.985 | 59.341 |
| NaNO3 | 1.695 | 19.941 |
| Na2S2O3 x 5 H2O | 0.399 | 1.607 |
| MgCl2 x 6 H2O | 0.199 | 0.981 |
| KCl | 0.1 | 1.337 |
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