1231
1231 .
HALOPLASMA CONTRACTILE MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Haloplasma contractile DSM 18853
This strain uses the standard medium without modifications.
Growth conditions: 35 °C
Main sol. 1231 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 80.0 | g | 80 | 1368.93 | |
| KH2PO4 | 0.5 | g | 0.5 | 3.674 | |
| Yeast extract | 0.2 | g | 0.2 | - | |
| Peptone | 0.2 | g | 0.2 | - | |
| Starch | 5.0 | g | 5 | - | |
| Na2S x 9 H2O | 0.5 | g | 0.5 | 2.082 | |
| Resazurin | 0.5 | µg | 5.0e-7 | 2.2e-6 | |
| Artificial seawater | 250.0 | ml | - | - | |
| Distilled water | 750.0 | ml | - | - | |
| 1 Prepare the medium without the Na2S x 9 H2O by boiling and cooling under a nitrogen atmosphere. Adjust the pH to 6.5 - 7.0. Dispense into Hungate tubes or serum bottles fitted with butyl rubber stoppers. Autoclave and reduce the medium before use by adding the appropriate amount of Na2S x 9 H2O from a neutralised, sterile stock solution. | |||||
Equipment needed: Hungate tubes
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 27.70 | g | 27.7 | 473.99 | |
| MgSO4 x 7 H2O | 7.00 | g | 7 | 28.4 | |
| MgCl2 x 6 H2O | 5.50 | g | 5.5 | 27.053 | |
| KCl | 0.60 | g | 0.6 | 8.048 | |
| NaBr | 0.10 | g | 0.1 | 0.972 | |
| H3BO3 | 0.03 | g | 0.03 | 0.485 | |
| CaCl2 x 2 H2O | 1.50 | g | 1.5 | 10.203 | |
| SrCl2 x 6 H2O | 15.00 | mg | 0.015 | 0.056 | |
| KI | 0.05 | mg | 5.0e-5 | 1.9e-4 | |
| Double distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 5.5 - 6.0. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.5 - 7.0 |
| Equipment needed: | Autoclave, Hungate tubes |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Haloplasma contractile DSM 18853
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 6.925 | 118.497 |
| Starch | 5 | - |
| MgSO4 x 7 H2O | 1.75 | 7.1 |
| MgCl2 x 6 H2O | 1.375 | 6.763 |
| KH2PO4 | 0.5 | 3.674 |
| Show more | ||
