122
122 .
ACETIVIBRIO MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Acetivibrio thermocellus DSM 1313
This strain uses the standard medium without modifications.
Growth conditions: anaerobic
60 °C
Main sol. 122 #
ml
Equipment needed: Hungate tubes
Filter
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 1.30 | g | 1.3 | 9.838 | |
| MgCl2 x 6 H2O | 2.60 | g | 2.6 | 12.789 | |
| KH2PO4 | 1.43 | g | 1.43 | 10.508 | |
| K2HPO4 | 5.50 | g | 5.5 | 31.577 | |
| CaCl2 x 2 H2O | 0.13 | g | 0.13 | 0.884 | |
|
Na2-ß-glycerophosphate x 5 H2O
(MERCK 35675) |
6.00 | g | 6 | 19.601 | |
|
FeSO4 x 7 H2O
(0.1% w/v in 0.1 N H2SO4) |
1.10 | ml | 0.001 | 0.004 | |
|
L-Glutathione
(reduced) |
0.25 | g | 0.25 | 0.813 | |
| Yeast extract | 4.50 | g | 4.5 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| Cellobiose | 5.00 | g | 5 | 14.607 | |
|
Cellulose
(Avicel or MN 301) optional |
10.00 | g | 10 | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except cellobiose, sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Then adjust pH to 7.0 - 7.2, distribute under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Cellobiose is added to the sterile medium from an anoxic 10% (w/v) stock solution prepared under 100% N2 gas and sterilized by filtration. Some strains can be adapted to cellulose as substrate using 10.00 g/l cellulose (Avicel or MN 301, MACHEREY-NAGEL). | |||||
| 2 Note: A white precipitate forms after mixing the ingredients of this medium, but this has no negative effect on growth. | |||||
Metadata
| Last modified: | 07.10.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 - 7.2 |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 10 |
Acetivibrio thermocellus DSM 1313
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
|
Cellulose optional |
10 | - |
| Na2-ß-glycerophosphate x 5 H2O | 6 | 19.601 |
| K2HPO4 | 5.5 | 31.577 |
| Cellobiose | 5 | 14.607 |
| Yeast extract | 4.5 | - |
| Show more | ||
Cultivation metadata from 10 strains
| pH | 6 - 8 | 2 strains |
| pH | 8 - 10 | 1 strain |
| phyla | 1 | 10 strains |
| oxygen | anaerobe | 9 strains |
| temperature | thermophilic | 9 strains |