1224
1224 .
ECTOTHIORHODOSPIRA VARIABILIS MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Ectothiorhodospira variabilis DSM 21381
This strain uses the standard medium without modifications.
Growth conditions: 3-7 days
anaerobic in light
28 °C
Main sol. 1224 #
ml
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 50.0 | g | 50 | 855.578 | |
| Na2CO3 | 5.0 | g | 5 | 47.175 | |
| NaHCO3 | 15.0 | g | 15 | 178.557 | |
| KCl | 0.1 | g | 0.1 | 1.341 | |
| K2HPO4 | 0.5 | g | 0.5 | 2.871 | |
| MgCl2 x 6 H2O | 0.2 | g | 0.2 | 0.984 | |
| NH4Cl | 0.5 | g | 0.5 | 9.348 | |
| Na2S x 9 H2O | 0.5 | g | 0.5 | 2.082 | |
| Na2S2O3 x 5 H2O | 0.5 | g | 0.5 | 2.015 | |
| Yeast extract | 0.1 | g | 0.1 | - | |
| Na acetate | 0.5 | g | 0.5 | 6.095 | |
| Trace element solution SL-10 | 1.0 | ml | - | - | |
| Vitamin B12 | 20.0 | µg | 2.0e-5 | 1.5e-5 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Final pH 9.0 - 9.5 | |||||
| 2 Prepare and boil the medium in the absence of Na2S x 9 H2O, NaHCO3, Vitamin B12, CaCl2 x 2 H2O, MgCl2 x 6 H2O. Cool under a stream of nitrogen and add the remaining components, except vitamin B12 and Na2S2O3 x 5 H2O. Dispense into tubes or bottle fitted with rubber stoppers and autoclave. Add the vitamin B12 (filter sterilised) and Na2S2O3 x 5 H2O from sterile stock solutions. | |||||
Solution occurs in 266
other media (first defined in Medium 320).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 9.0 - 9.5 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Ectothiorhodospira variabilis DSM 21381
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 50 | 855.578 |
| NaHCO3 | 15 | 178.557 |
| Na2CO3 | 5 | 47.175 |
| NH4Cl | 0.5 | 9.348 |
| Na acetate | 0.5 | 6.095 |
| Show more | ||
