1208 .
BACILLUS ALKALIDIAZOTROPHICUS MEDIUM
What is this page?
Equipment needed:
Autoclave
Filter
Gassing station
Final gas composition:
100 % N2
read more
Final pH:
10.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaHCO3 | 8.00 | g | ||
|
|
Na2CO3 | 22.00 | g | ||
|
|
NaCl | 6.00 | g | ||
|
|
K2HPO4 | 1.00 | g | ||
|
|
NH4Cl | 0.20 | g | ||
|
|
MgCl2 x 6 H2O | 0.20 | g | ||
|
|
Yeast extract | 0.05 | g | ||
|
|
Na2SO4 | 2.80 | g | ||
|
|
Trace element solution SL-11 | 1.00 | ml | ||
|
|
Selenite-tungstate solution | 1.00 | ml | ||
|
|
Wolin's vitamin solution | 10.00 | ml | ||
|
|
Glucose | 2.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve ingredients except ammonium chloride, magnesium chloride, yeast extract, trace elements, vitamins, boil medium for 1 min, then cool to room temperature under N2 gas atmosphere. Dispense under same gas atmosphere in culture vessels and autoclave. After sterilization add ammonium chloride, magnesium chloride, yeast extract, trace elements, vitamins, from sterile, anoxic stock solution prepared under N2. The vitamin solution should be sterilized by filtration. Adjust pH of final medium to 10. | |||||
Trace element solution SL-11 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2-EDTA x 2 H2O | 5.2 | g | ||
|
|
FeCl2 x 4 H2O | 1.5 | g | ||
|
|
ZnCl2 | 70.0 | mg | ||
|
|
MnCl2 x 4 H2O | 100.0 | mg | ||
|
|
H3BO3 | 6.0 | mg | ||
|
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
CuCl2 x 2 H2O | 2.0 | mg | ||
|
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml. | |||||
Selenite-tungstate solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaOH | 0.5 | g |
|
|
Na2SeO3 x 5 H2O | 3.0 | mg |
|
|
Na2WO4 x 2 H2O | 4.0 | mg |
|
|
Distilled water | 1000.0 | ml |
Wolin's vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
|
Pyridoxine hydrochloride | 10.0 | mg |
|
|
Thiamine HCl | 5.0 | mg |
|
|
Riboflavin | 5.0 | mg |
|
|
Nicotinic acid | 5.0 | mg |
|
|
Calcium D-(+)-pantothenate | 5.0 | mg |
|
|
Vitamin B12 | 0.1 | mg |
|
|
p-Aminobenzoic acid | 5.0 | mg |
|
|
(DL)-alpha-Lipoic acid | 5.0 | mg |
|
|
Distilled water | 1000.0 | ml |