11
11 .
MRS MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Lactobacillus delbrueckii subsp. lactis DSM 20355
* Modification: Plus vitamin solution (see medium 141)
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 37 °C
Main sol. 11 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Casein peptone
(tryptic digest) |
10.00 | g | 10 | - | |
| Meat extract | 10.00 | g | 10 | - | |
| Yeast extract | 5.00 | g | 5 | - | |
| Glucose | 20.00 | g | 20 | 111.012 | |
| Tween 80 | 1.00 | g | 1 | 0.764 | |
| K2HPO4 | 2.00 | g | 2 | 11.483 | |
| Na-acetate | 5.00 | g | 5 | 60.951 | |
| (NH4)3 citrate | 2.00 | g | 2 | 8.223 | |
| MgSO4 x 7 H2O | 0.20 | g | 0.2 | 0.811 | |
| MnSO4 x H2O | 0.05 | g | 0.05 | 0.296 | |
| Wolin's vitamin solution* | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 6.2 - 6.5. | |||||
Solution occurs in 53
other media (first defined in Medium 141).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium D-(+)-pantothenate | 5.0 | mg | 0.005 | 0.021 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| (DL)-alpha-Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.2 - 6.5 |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Glucose | 20 | 111.012 |
| Casein peptone | 10 | - |
| Meat extract | 10 | - |
| Na-acetate | 5 | 60.951 |
| Yeast extract | 5 | - |
| Show more | ||
Cultivation metadata from 487 strains
| pH | 6 - 8 | 35 strains |
| pH | 4 - 6 | 21 strains |
| pH | 8 - 10 | 14 strains |
| pH | 2 - 4 | 11 strains |
| phyla | 5 | 481 strains |
| Show more | ||