This medium was extracted automatically and has not been curated by experts, yet.
This strain uses the standard medium without modifications.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 30.00 | g | 30 | 513.347 | |
Na2CO3 | 2.76 | g | 2.76 | 26.041 | |
NaHCO3 | 24.00 | g | 24 | 285.69 | |
KCl | 0.20 | g | 0.2 | 2.683 | |
K2HPO4 | 0.20 | g | 0.2 | 1.148 | |
MgCl2 x 6 H2O | 0.10 | g | 0.1 | 0.492 | |
NH4Cl | 1.00 | g | 1 | 18.695 | |
Na2S x 9 H2O | 0.40 | g | 0.4 | 1.665 | |
Yeast extract | 0.50 | g | 0.5 | - | |
D-Glucose | 5.00 | g | 5 | 27.753 | |
Vitamin solution | 2.00 | ml | - | - | |
Trace element solution | 1.00 | ml | - | - | |
Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
Distilled water | 1000.00 | ml | - | - | |
1 Adjust to pH 9.4 | |||||
2 Prepare the medium without the Na2S, yeast extract, glucose, Na2CO3, NaHCO3 and vitamins. Heat the medium in a boiling water bath and cool under a stream of nitrogen. Add the Na2CO3, and NaHCO3 to the cooled medium and check the pH. Dispense into appropriate vessels (Hungate tubes or serum bottles) and autoclave. To the sterile medium add Na2S, yeast extract, and glucose from autoclaved stock solutions. The vitamin solution is filter sterilised and added to the sterilised medium. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine-HCl x 2 H2O | 5.0 | mg | 0.005 | 0.013 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
D-Calcium pantothenate | 5.0 | mg | 0.005 | - |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
MnCl2 x 4 H2O | 720 | mg | 3.6 | 18.191 |
Fe(NH4)2(SO4)2 x 12 H2O | 400 | mg | 2 | - |
FeSO4 x 7 H2O | 200 | mg | 1 | 3.597 |
CoCl2 x 6 H2O | 200 | mg | 1 | 7.702 |
ZnSO4 x 7 H2O | 200 | mg | 1 | 3.478 |
Na2MoO4 x 2 H2O | 20 | mg | 0.1 | 0.413 |
NiCl2 | 100 | mg | 0.5 | 3.858 |
CuSO4 x 5 H2O | 20 | mg | 0.1 | 0.401 |
AlK(SO4)2 x 12 H2O | 20 | mg | 0.1 | 0.211 |
H3BO3 | 20 | mg | 0.1 | 1.617 |
HCl | 5 | ml | - | - |
Distilled water | 200 | ml | - | - |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 9.4 |
Equipment needed: | Autoclave, Filter, Hungate tubes |
Edit in Medium builder |
All strains for this medium: | 1 |
No modifications necessary for this strain.
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 30 | 513.347 |
NaHCO3 | 24 | 285.69 |
D-Glucose | 5 | 27.753 |
Na2CO3 | 2.76 | 26.041 |
NH4Cl | 1 | 18.695 |
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