MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 1105 . NITRILIRUPTOR ALKALIPHILUS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Medium for Alkalihalobacterium alkalinitrilicum DSM 22532

This strain uses the standard medium without modifications.

Growth conditions: 28 °C

Main sol. 1105 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 22.0 g 22 207.571
NaHCO3 8.0 g 8 95.23
NaCl 6.0 g 6 102.669
K2HPO4 0.5 g 0.5 2.871
Distilled water 1000.0 ml - -
1 Dissolve in this order in 1L preferably MilliQ water. Sterilize in closed bottles at 120°C 20 min. After cooling, usually there will be some precipitate on the bottom. That´s why it is handy to have another empty 1L bottle to decant clear top solution into it.
2 After sterilization add from concentrated steril stock solutions:
Trace metals solution (Pfennig & Lippert, 1966) 1.0 ml - -
MgSO4 x 7 H2O
(20 %) 		1.0 ml 0.2 0.811
Na2S2O3 x 5 H2O
(2 M, pH 10) 		10.0 μl - -
Vitamin B12
(0.01 %) 		1.0 ml 1.0e-4 7.4e-5
Isobutyramide
(1 M, 8.7 g in 100 ml H2O dest) 		10.0 ml 0.871 10
3 Trace metals, MgSO4 x 7 H2O and Na2S2O3 x 5 H2O solution you can autoclave
4 Vitamin B12 and Isobutyramide solution you have to filter-sterilize

Sodium thiosulfate solution (available) #

100
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S2O3 50 % 500 1166.82
1 50% solution of Na2S2O3 (Fluka), pH 9-10 >> filter sterilize or 110°C 20 min

Trace metals solution (Pfennig & Lippert, 1966) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA
(Trilon B) 		5.00 g 5 17.109
FeSO4 x 7 H2O 2.00 g 2 7.194
ZnSO4 0.10 g 0.1 0.62
MnCl2 0.03 g 0.03 0.238
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 0.01 g 0.01 0.074
NiCl2 x 2 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.02 g 0.02 0.083
Distilled water 1000.00 ml - -
1 pH final 3-4 by HCl

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 10.0
Equipment needed: Autoclave, Filter
Edit in Medium builder

Associated strains

All strains for this medium: 2
Alkalihalobacterium alkalinitrilicum DSM 22532

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2CO3 22 207.571
NaHCO3 8 95.23
NaCl 6 102.669
Isobutyramide 0.871 10
K2HPO4 0.5 2.871
Show more
CSV
JSON

Cultivation metadata from 2 strains BacDive

pH 8 - 10 1 strain
pH 10 - 12 1 strain
phyla 2 2 strains
oxygen aerobe 1 strain
temperature mesophilic 2 strains