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DSMZ

DSMZ 1105 . NITRILIRUPTOR ALKALIPHILUS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Alkalihalobacterium alkalinitrilicum DSM 22532

This strain uses the standard medium without modifications.

Growth conditions: 28 °C

Main sol. 1105 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 22.0 g 22 207.571
NaHCO3 8.0 g 8 95.23
NaCl 6.0 g 6 102.669
K2HPO4 0.5 g 0.5 2.871
Distilled water 1000.0 ml - -
1 Dissolve in this order in 1L preferably MilliQ water. Sterilize in closed bottles at 120°C 20 min. After cooling, usually there will be some precipitate on the bottom. That´s why it is handy to have another empty 1L bottle to decant clear top solution into it.
2 After sterilization add from concentrated steril stock solutions:
Trace metals solution (Pfennig & Lippert, 1966) 1.0 ml - -
MgSO4 x 7 H2O
(20 %) 		1.0 ml 0.2 0.811
Na2S2O3 x 5 H2O
(2 M, pH 10) 		10.0 μl - -
Vitamin B12
(0.01 %) 		1.0 ml 1.0e-4 7.4e-5
Isobutyramide
(1 M, 8.7 g in 100 ml H2O dest) 		10.0 ml 0.871 10
3 Trace metals, MgSO4 x 7 H2O and Na2S2O3 x 5 H2O solution you can autoclave
4 Vitamin B12 and Isobutyramide solution you have to filter-sterilize

Sodium thiosulfate solution (available) #

100
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S2O3 50 % 500 1166.82
1 50% solution of Na2S2O3 (Fluka), pH 9-10 >> filter sterilize or 110°C 20 min

Trace metals solution (Pfennig & Lippert, 1966) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA
(Trilon B) 		5.00 g 5 17.109
FeSO4 x 7 H2O 2.00 g 2 7.194
ZnSO4 0.10 g 0.1 0.62
MnCl2 0.03 g 0.03 0.238
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 0.01 g 0.01 0.074
NiCl2 x 2 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.02 g 0.02 0.083
Distilled water 1000.00 ml - -
1 pH final 3-4 by HCl

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 10.0
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 2
Alkalihalobacterium alkalinitrilicum DSM 22532

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2CO3 22 207.571
NaHCO3 8 95.23
NaCl 6 102.669
Isobutyramide 0.871 10
K2HPO4 0.5 2.871
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Cultivation metadata from 2 strains BacDive

pH 8 - 10 1 strain
pH 10 - 12 1 strain
phyla 2 2 strains
oxygen aerobe 1 strain
temperature mesophilic 2 strains