Main sol. 1105
Internal Solution-ID: S2222
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2CO3 | 22.0 | g | 22 | 207.571 | |
| NaHCO3 | 8.0 | g | 8 | 95.23 | |
| NaCl | 6.0 | g | 6 | 102.669 | |
| K2HPO4 | 0.5 | g | 0.5 | 2.871 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve in this order in 1L preferably MilliQ water. Sterilize in closed bottles at 120°C 20 min. After cooling, usually there will be some precipitate on the bottom. That´s why it is handy to have another empty 1L bottle to decant clear top solution into it. | |||||
| 2 After sterilization add from concentrated steril stock solutions: | |||||
| Trace metals solution (Pfennig & Lippert, 1966) | 1.0 | ml | - | - | |
|
MgSO4 x 7 H2O
(20 %) |
1.0 | ml | 0.2 | 0.811 | |
|
Na2S2O3 x 5 H2O
(2 M, pH 10) |
10.0 | μl | - | - | |
|
Vitamin B12
(0.01 %) |
1.0 | ml | 1.0e-4 | 7.4e-5 | |
|
Isobutyramide
(1 M, 8.7 g in 100 ml H2O dest) |
10.0 | ml | 0.871 | 10 | |
| 3 Trace metals, MgSO4 x 7 H2O and Na2S2O3 x 5 H2O solution you can autoclave | |||||
| 4 Vitamin B12 and Isobutyramide solution you have to filter-sterilize | |||||
Media with this solution
| 1105 | NITRILIRUPTOR ALKALIPHILUS MEDIUM |