1091
1091 .
HALOQUADRATUM WALSBYI MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Natronomonas moolapensis DSM 18674
This strain uses the standard medium without modifications.
Growth conditions: 37 °C
Main sol. 1091 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 195.00 | g | 195 | 3336.76 | |
| MgSO4 x 7 H2O | 50.00 | g | 50 | 202.86 | |
| MgCl2 x 6 H2O | 35.00 | g | 35 | 172.157 | |
| KCl | 5.00 | g | 5 | 67.069 | |
| NaHCO3 | 0.25 | g | 0.25 | 2.976 | |
| NaNO3 | 1.00 | g | 1 | 11.765 | |
| CaCl2 x 2 H2O | 0.50 | g | 0.5 | 3.401 | |
| KH2PO4 | 0.05 | g | 0.05 | 0.367 | |
| NH4Cl | 0.03 | g | 0.03 | 0.561 | |
|
Tris buffer
(1M) |
20.00 | ml | 2.423 | 20 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Add after autoclaving: | |||||
| Yeast extract | 0.05 | g | 0.05 | - | |
| Sodium pyruvate | 1.00 | g | 1 | 9.088 | |
| 2 Final pH 7.4 | |||||
| 3 Yeast extract and sodium pyruvate are added from sterile (autoclaved) stock solutions to the respective concentrations. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Archaea |
| Medium type: | Complex medium |
| Final pH: | 7.4 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 10 |
Natronomonas moolapensis DSM 18674
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 195 | 3336.76 |
| MgSO4 x 7 H2O | 50 | 202.86 |
| MgCl2 x 6 H2O | 35 | 172.157 |
| KCl | 5 | 67.069 |
| Tris buffer | 2.423 | 20 |
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