1029
1029 .
BBL ACTINOMYCES BROTH
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Actinomyces sp. DSM 100376
This strain uses the standard medium without modifications.
Growth conditions: 37 °C
Main sol. 1029 #
ml
Equipment needed: Autoclave
Wheaton tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| BBL Actinomyces broth (BBL 210920) | 57 | g | 57 | - | |
| Distilled water | 1000 | ml | - | - | |
| 1 final pH 6.9 | |||||
| 2 Distribute into screw-capped Wheaton tubes. For anaerobic handling prepare and distribute into Hungate tubes by using the Hungate technique. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Potassium Phosphate | 15.00 | g | 15 | 70.666 |
| Ammonium Sulfate | 1.00 | g | 1 | - |
| Magnesium sulfate | 0.20 | g | 0.2 | 1.662 |
| Calcium Chloride | 0.01 | g | 0.01 | 0.068 |
| Infusion Broth | 25.00 | g | 25 | - |
| Dextrose | 5.00 | g | 5 | 25.231 |
| L-Cysteine HCl | 1.00 | g | 1 | - |
| Pancreatic digest of casein | 4.00 | g | 4 | - |
| Yeast extract | 5.00 | g | 5 | - |
|
Starch
(soluble) |
1.00 | g | 1 | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.9 |
| Equipment needed: | Autoclave, Wheaton tubes |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 9 |
| Strains with modifications: | 1 |
Actinomyces sp. DSM 100376
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Infusion Broth | 1.425 | - |
| Potassium Phosphate | 0.855 | 4.028 |
| Dextrose | 0.285 | 1.438 |
| Yeast extract | 0.285 | - |
| Pancreatic digest of casein | 0.228 | - |
| Show more | ||
