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DSMZ

DSMZ 1001 . BASAL MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1001 #

ml
Equipment needed: Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 2.50 g 2.5 29.759
NH4Cl 0.25 g 0.25 4.674
NaH2PO4 x H2O 0.60 g 0.6 5.001
KCl 0.10 g 0.1 1.341
Vitamin mixture 10.00 ml - -
Mineral mixture 10.00 ml - -
Distilled water 980.00 ml - -
1 This medium should not be exposed to direct sunlight! Add all basal medium ingredients. Bring the final volume of the medium to 1.0 liter. Dispense to appropriate culture containers. Bubble the medium with 80:20 N2:CO2 (final pH sould be 6.8 to 7.0) - approximately 10 ml of media (anaerobic culture tube) should be gassed for 5 min in the aqueous phase (bubbled) and the headspace gassed for one minute prior to sealing the container. Sterilize per usual procedure. Add electron donor (Acetate-final conc. of 10 mM-recipe below) and electron acceptor (Fe(III)NTA-final conc. of 10 mM-recipe below) from sterile, anaerobic stock solutions using a sterile syringe and needle flushed with anaerobic gas. Store out of direct light.

1.0 M Acetate Stock Solution #

100
Equipment needed: Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
Sodium acetate 13.6 g 136 1657.93
Distilled water 100.0 ml - -
1 Add 13.6 g sodium acetate to ca. 80 ml distilled water. Bring to final volume of 100 ml. Bubble with anaerobic gas (nitrogen) for 45 min. Seal and sterilize.

500 mM Fe(III)NTA Stock solution #

300
Equipment needed: Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 8.2 g 27.333 325.37
Nitrilotriacetic acid 12.8 g 42.667 223.222
FeCl3 x 6 H2O 13.5 g 45 166.484
1 Add 8.2 g NaHCO3 to ca. 70 ml distilled water. Add 12.8 g of Na3Nitrilotriacetic acid (NTA) to ca. 70 ml distilled water. Add 13.5 g FeCl3 x 6 H2O to ca 70 ml distilled water. Adjust pH to 6.5 using 10N NaOH. Bring solution to final volume of 100 ml. Ingredients will go into solution after stirring for ca. 15 min. Bubble with anaerobic gas (N2) for 45 min and then filter sterilize (0.2 µm filter) into a sterile, anaerobic, serum bottle.

Vitamin mixture #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Riboflavin 5.0 mg 0.005 0.013
Thiamine 5.0 mg 0.005 0.019
Nicotinic acid 5.0 mg 0.005 0.041
Pantothenic acid 5.0 mg 0.005 0.023
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Thioctic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Mineral mixture #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
NTA 1.500 g 1.5 7.848
MgSO4 3.000 g 3 24.923
MnSO4 x H2O 0.500 g 0.5 2.958
NaCl 1.000 g 1 17.112
FeSO4 x 7 H2O 0.100 g 0.1 0.36
CaCl2 x 2 H2O 0.100 g 0.1 0.68
CoCl2 x 6 H2O 0.100 g 0.1 0.77
ZnCl2 0.130 g 0.13 0.954
CuSO4 x 5 H2O 0.010 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.010 g 0.01 0.021
H3BO3 0.010 g 0.01 0.162
Na2MoO4 0.025 g 0.025 0.121
NiCl2 x 6 H2O 0.024 g 0.024 0.185
Na2WO4 x 2 H2O 0.025 g 0.025 0.076
Distilled water 1000.000 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.9
Equipment needed: Anaerobic workbench, Filter
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 2.5 29.759
NaH2PO4 x H2O 0.6 5.001
NH4Cl 0.25 4.674
KCl 0.1 1.341
MgSO4 0.03 0.249
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain