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DSMZ

DSMZ 1001 . BASAL MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1001 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 2.50 g 2.5 29.759
NH4Cl 0.25 g 0.25 4.674
NaH2PO4 x H2O 0.60 g 0.6 5.001
KCl 0.10 g 0.1 1.341
Mineral mixture 10.00 ml - -
Distilled water 990.00 ml - -
1 Add all basal medium ingredients without vitamins.
2 Sparge medium with 80% N2 and 20% CO2 gas mixture for 10 min Bubble the medium with N2:CO2 (80:20) to make anoxic.
3 Adjust pH to 6.8-7.0.
4 Dispense into serum bottles (using 10ml medium in 100 ml bottles with N2:CO2 headspace).
5 Autoclave at 121 °C for 15 min.
6 After autoclaving, add the following solutions to a final volume of 10ml/L each.
NaHCO3
(1M in water, sterile, anaerobic) 		10.00 ml/l 0.84 10
Na acetate
(1M in water, sterile, anaerobic) 		10.00 ml/l 0.82 10
FeCl3 x 6 H2O
(0,5 M in water, sterile, anaerobic) 		10.00 ml/l 13.515 50
Wolin's vitamin solution 10.00 ml - -
7 Note: This medium should not be exposed to direct sunlight! Store out of direct light.

Mineral mixture #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
NTA 1.500 g 1.5 7.848
MgSO4 3.000 g 3 24.923
MnSO4 x H2O 0.500 g 0.5 2.958
NaCl 1.000 g 1 17.112
FeSO4 x 7 H2O 0.100 g 0.1 0.36
CaCl2 x 2 H2O 0.100 g 0.1 0.68
CoCl2 x 6 H2O 0.100 g 0.1 0.77
ZnCl2 0.130 g 0.13 0.954
CuSO4 x 5 H2O 0.010 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.010 g 0.01 0.021
H3BO3 0.010 g 0.01 0.162
Na2MoO4 0.025 g 0.025 0.121
NiCl2 x 6 H2O 0.024 g 0.024 0.185
Na2WO4 x 2 H2O 0.025 g 0.025 0.076
Distilled water 1000.000 ml - -

Wolin's vitamin solution #

1000
Solution occurs in 54 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 0.021
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 08.01.25
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.9
Equipment needed: Autoclave
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
FeCl3 x 6 H2O 13.515 50
NaHCO3 0.84 10
Na acetate 0.82 10
NaH2PO4 x H2O 0.6 5.001
NH4Cl 0.25 4.674
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain