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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.
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Current criteria: poly-ß-hydroxybutyric acid
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474 MEDIUM WITH POLYHYDROXYBUTYRIC ACID AS CARBON SOURCE
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 16 | 47.572 |
| Na2HPO4 | 0.244 | 1.719 |
| poly-ß-hydroxybutyric acid | 0.198 | - |
| KH2PO4 | 0.152 | 1.117 |
| (NH4)2SO4 | 0.05 | 0.378 |
| MgSO4 x 7 H2O | 0.02 | 0.081 |
| CaCl2 x 2 H2O | 0.005 | 0.034 |
| EDTA | 5.0e-4 | 0.002 |
| FeSO4 x 7 H2O | 2.0e-4 | 7.2e-4 |
| H3BO3 | 3.0e-5 | 4.9e-4 |
| CoCl2 x 6 H2O | 2.0e-5 | 1.5e-4 |
| ZnSO4 x 7 H2O | 1.0e-5 | 3.5e-5 |
| MnCl2 x 4 H2O | 3.0e-6 | 1.5e-5 |
| Na2MoO4 x 2 H2O | 3.0e-6 | 1.2e-5 |
| NiCl2 x 6 H2O | 2.0e-6 | 1.5e-5 |
| CuCl2 x 2 H2O | 1.0e-6 | 5.9e-6 |
| Distilled water | - | - |
Solutions:
- 10 ml Trace element solution SL-4
- 6.6 ml Poly-ß-hydroxybutyric acid solution 3%(w/v)
- 100 ml Trace element solution SL-6